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Mechanismofboneresorption inmiddleearcholesteatoma inthe light of H^+ concentration and H^+-sensing Proteins

H^浓度和H^敏感蛋白对中耳胆脂瘤骨吸收机制的影响

基本信息

批准号：
22591893
负责人：
SUZUKI Hideaki
金额：
$ 2.25万
依托单位：
University of Occupational and Environmental Health, Japan
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Objective:The etiopathology of bone resorption in cholesteatoma is unclear. We studied pH inmiddle ear cholesteatoma tissue and the permeability of the cholesteatoma epithelium in an attempt to elucidate the mechanism of bone resorption in this disease.Methods:Cholesteatoma tissue was collected from patients with primary acquired middle ear cholesteatoma. The pH of the keratin debris of cholesteatoma was measured using a pH meter. The cholesteatoma epithelium was examined under a confocal laser scanning microscope, and under a transmission electron microscope. Expression of filaggrin in the cholesteatoma tissue was explored by fluorescence immunohistochemistry and by quantitative reverse transcription-polymerase chain reaction.Results: The pH of the keratin debris of cholesteatoma was acidic. The pH of the basal layer of the cholesteatoma epithelium was significantly lower than that of the antrum mucosa. Transmission electron microscope showed distinct penetration of lanthanum in the intercellular space of the basal, spinous and granular layers of the cholesteatoma epithelium, but only a small amount of lanthanum in the granular layer in the normal skin. The expression of filaggrin nlRNA was significantly lower in the cholesteatoma tissue than in the normal skin.Conclusions: These results indicate that acid leakage through the cholesteatonla epitheliunl probably participates in the resorption of the underlying bone structure. The increased permeability of the cholesteatoma epitheliunl may be explained by a decrease in filaggrin expression.

目的：中耳炎性骨吸收的发病机制尚不清楚。为探讨中耳胆脂瘤骨吸收的机制，我们对中耳胆脂瘤组织的pH值和胆脂瘤上皮的通透性进行了研究。使用pH计测量中耳炎瘤的角蛋白碎片的pH。在激光共聚焦显微镜和透射电子显微镜下观察中耳乳突瘤上皮。采用荧光免疫组化和定量逆转录-聚合酶链反应（RT-PCR）方法检测角蛋白丝聚蛋白在中耳乳突瘤组织中的表达。中耳乳突瘤上皮基底层pH值明显低于胃窦粘膜。透射电镜下可见镧在正常皮肤的颗粒层、棘层和基底层的细胞间隙中有明显的渗透。丝聚蛋白nlRNA的表达显着降低，在lapteatoma组织比在正常skin.Conclusions：这些结果表明，酸渗漏通过lapteatonla epitheliunl可能参与下面的骨结构的吸收。中耳炎上皮细胞通透性增加可能是由于聚丝蛋白表达减少所致。