CLONING OF LAMININ GENE BINDING PROTEIN

在高血压、心肌病和糖尿病等疾病中，层粘连蛋白在病理上增加。我们已经研究了层粘连蛋白B2链基因启动子的转录机制，发现在层粘连蛋白启动子中有一个基序，它与启动子结合并激活层粘连蛋白转录。根据本研究项目，我们利用酵母单杂交系统对小鼠cDNA文库进行了筛选，以克隆与层粘连蛋白基因启动子结合并激活的转录因子。因此，我们克隆了三个与EMSA体外结合的转录因子，并通过荧光素酶报告基因分析激活了其启动子；克隆了未知的TFE-3、RTEF和K46克隆，并与层粘连蛋白基因启动子结合。我们现在正在准备使用这些结果提交这篇论文。

Laminin increases pathologically in the diseases as hypertension, cardiomyopathy and diabetes mellitus. We have been examined the transcription mechanisms of laminin B2 chain gene promoter and found out one motif, in the laminin promoter, which binds the promoter and activates laminin transcription.According to the research project, we performed the screening of the mouse cDNA library using yeast one-hybrid system to clone a transcription factor which binds and activates laminin gene promoter. As a result, we cloned three transcription factors which bind in vitro EMSA assay and activate its promoter using luciferase reporter assay.We cloned TFE-3 , RTEF and K46 clone which is not known, and all three clones bind laminin gene promoter. We are now preparing for submission of the paper using those results.