CLONING OF LAMININ GENE BINDING PROTEIN

层粘连蛋白基因结合蛋白的克隆

• 批准号: 11670711
• 负责人: SUZUKI Hideaki
• 金额: $ 1.86万
• 依托单位: JIKEI UNIVERSITY SCHOOL of MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670711/
• 关键词: laminin; transcription factor; promoter; cloniong; one-hybrid system; luciferase; yeast; plasmid

Laminin increases pathologically in the diseases as hypertension, cardiomyopathy and diabetes mellitus. We have been examined the transcription mechanisms of laminin B2 chain gene promoter and found out one motif, in the laminin promoter, which binds the promoter and activates laminin transcription.According to the research project, we performed the screening of the mouse cDNA library using yeast one-hybrid system to clone a transcription factor which binds and activates laminin gene promoter. As a result, we cloned three transcription factors which bind in vitro EMSA assay and activate its promoter using luciferase reporter assay.We cloned TFE-3 , RTEF and K46 clone which is not known, and all three clones bind laminin gene promoter. We are now preparing for submission of the paper using those results.

层粘连蛋白在高血压、心肌病、糖尿病等疾病中呈病理性增高。本研究对层粘连蛋白B2链基因启动子的转录机制进行了研究，发现层粘连蛋白B2链基因启动子中存在一个与启动子结合并激活层粘连蛋白转录的基序，并根据本研究项目，利用酵母单杂交系统筛选小鼠cDNA文库，克隆了一个与层粘连蛋白基因启动子结合并激活层粘连蛋白基因启动子的转录因子。结果，我们克隆了三个转录因子，它们在体外EMSA试验中结合，并在荧光素酶报告试验中激活其启动子，我们克隆了TFE-3、RTEF和未知的K46克隆，这三个克隆都结合层粘连蛋白基因启动子。我们现在正准备提交使用这些结果的文件。

项目成果

Suzuki H, et. al.: "Inducible transcriptional activity of bcn-1 element from laminin γ1-chain gene promoter in renal and nonrenal cells."Am.J.Physiol.. 275(44). F518-26 (1998)

Suzuki H 等人：“肾细胞和非肾细胞中层粘连蛋白 γ1 链基因启动子的 bcn-1 元件的诱导转录活性”。Am.J.Physiol.. 275(44) F518-26 (1998)。

Suzuki H, et.al.: "Treatment of glomerular epithelial and mesangial cells with IL-1B and PMA activastes proteins and bind to a motif from the laminin B2 chain promoter."J.Am.Soc.Nephrology.. 5(3). 820 (1994)

Suzuki H 等人：“用 IL-1B 和 PMA 治疗肾小球上皮细胞和系膜细胞可激活蛋白质并与层粘连蛋白 B2 链启动子的基序结合。”J.Am.Soc.Nephrology.. 5(3)

Suzuki H, et.al.: "Recognition of two unrelated transcription factors, TFE3 and RTFE/TFER1, by the laminin γ1 chain gene promoter bcn-1 element."J.Am.Soc.Nephrol.. 10. 581 (1999)

Suzuki H 等人：“层粘连蛋白 γ1 链基因启动子 bcn-1 元件对两个不相关的转录因子 TFE3 和 RTFE/TFER1 的识别。”J.Am.Soc.Nephrol.. 10. 581 (1999)

Suzuki H, et.al.: "Recognition of two unrelated transcription factors, TFE3 and RTFE/TFFR1, by the Iaminin γ1 chain gone promoter bcn-1 element."J,Am.Soc.Nephrol.. 10. 581 (1999)

Suzuki H 等人：“通过层粘连蛋白 γ1 链消失启动子 bcn-1 元件识别两个不相关的转录因子 TFE3 和 RTFE/TFFR1。”J,Am.Soc.Nephrol.. 10. 581 (1999)

Suzuki H, et.al.: "Activation of a DNA-binding protein recognized by a transcriptinal element, bcn-1, from the laminin B2 chain gene promoter."J.Biol.Chem. 271. 18981-18988 (1996)

Suzuki H 等人：“由来自层粘连蛋白 B2 链基因启动子的转录元件 bcn-1 识别的 DNA 结合蛋白的激活。”J.Biol.Chem。