development of a novel beta-cell replacement therapy using the adipose-tissue derived stem cell(ADSC)

使用再生药物的β细胞替代疗法有望治愈1型糖尿病。本研究在建立链脲佐菌素诱导的糖尿病裸小鼠人脂肪组织来源干细胞移植模型的基础上，研究了hADSC对体内有功能的胰岛样细胞分化分化的影响，体内hADSC对受损胰岛细胞的归巢作用，以及hADSC对受损胰岛细胞的细胞保护作用。HADSC移植到胰腺本身后，可分化为功能性胰岛样细胞，改善糖耐量异常。此外，虽然在体外，hADSC具有无细胞接触的胰岛细胞保护作用，但在体内，静脉注射hADSC对损伤的胰岛细胞的归巢作用较差。

β-cell replacement therapy using regeneration medicines is expected as a cure for type 1 diabetes. This time, after the human adipose-tissue derived stem cell(hADSC) transplant model using streptozotocin-induced diabetes nude mice will be created, we investigated the differentiated and derived impact of hADSC to the functional islet like cell in vivo, the homing effect of hADSC on injured islet cell in vivo and the cell protective effects of hADSC on injured islet cell in vitro. hADSC transplanted to pancreas itself was differentiated to functional islet like cell, and improved the abnormal glucose tolerance. Moreover, although, in vitro, hADSC had an islet cell protection effect without the cell-cell contact, in vivo, the homing effect on the injured islet cell by the intravenous injection was poor.