Effects of local anesthetics on apoptosis in tumor cells.

局部麻醉药对肿瘤细胞凋亡的影响。

• 批准号: 22791446
• 负责人: TAMURA Ryuji
• 金额: $ 2.75万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791446/
• 关键词: 麻酔学; 局所麻酔薬; 腫瘍特異性; アポトーシス; ミトコンドリア; リドカイン; HL60; フリーラジカル

The aim of this study was to clarify the local anesthetics that induce apoptosis or necrosis and their induction-related factors.Methods : Apoptosis and necrosis were analyzed by double-staining assay with propidium iodide(PI) and annexin-V and were measured by flow cytometry(FACS). DNA fragmentation was used for the analysis of apoptosis.Conclusion : The results indicate that local anesthetics with high lipophilicity are highly toxic and induce mainly necrosis, while local anesthetics with low pKa induce more apoptosis.

本研究的目的是阐明局麻药诱导细胞凋亡或坏死及其诱导相关因素。方法：采用碘化丙啶（PI）和膜联蛋白v （annexin-V）双染色法分析细胞凋亡和坏死，流式细胞术（FACS）检测细胞凋亡和坏死。采用DNA片段法分析细胞凋亡。结论：高亲脂性局麻药毒性大，主要诱导细胞坏死，低pKa局麻药诱导细胞凋亡较多。

项目成果

Capsaicin Indirectly Suppresses Voltage-Gated Na+ Currents Through TRPV1 in Rat Dorsal Root Ganglion Neurons

• DOI: 10.1213/ane.0b013e318204ea5b
• 发表时间: 2011-03-01
• 期刊: ANESTHESIA AND ANALGESIA
• 影响因子: 5.7
• 作者: Onizuka, Shin;Yonaha, Tetsu;Tsuneyoshi, Isao
• 通讯作者: Tsuneyoshi, Isao