Analysis of analgesic action of remifentanil through dimerized mu-delta opioid receptors

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Baby hamster kidney cells transfected with Venus-fused mu opioid receptors and Cerulean-fused delta opioid receptors were treated with remifentanil. To investigate the remifentanil-induced internalization and resensitization of the heterodimers, cells were fixed and examined by confocal microscopy after treatment of remifentanil. Fluorescence resonance energy transfer analysis was performed to confirm the heterodimerization of mu opioid receptors and delta opioid receptors. Remifentanil caused internalization of mu-delta heterodimers in a dose-dependent manner. Compared to our previous study using baby hamster kidney cells transfected with mu opioid receptors alone, baby hamster kidney cells transfected with mu opioid receptors and delta opioid receptors were more efficiently returned to plasma membrane after the remifentanil-induced internalization.