Analysis of analgesic action of remifentanil through dimerized mu-delta opioid receptors

瑞芬太尼通过二聚μ-δ阿片受体的镇痛作用分析

• 批准号: 22791441
• 负责人: MURATA Hiroaki
• 金额: $ 2.5万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791441/
• 关键词: オピオイド受容体; 二量体; レミフェンタニル; 急性耐性

Baby hamster kidney cells transfected with Venus-fused mu opioid receptors and Cerulean-fused delta opioid receptors were treated with remifentanil. To investigate the remifentanil-induced internalization and resensitization of the heterodimers, cells were fixed and examined by confocal microscopy after treatment of remifentanil. Fluorescence resonance energy transfer analysis was performed to confirm the heterodimerization of mu opioid receptors and delta opioid receptors. Remifentanil caused internalization of mu-delta heterodimers in a dose-dependent manner. Compared to our previous study using baby hamster kidney cells transfected with mu opioid receptors alone, baby hamster kidney cells transfected with mu opioid receptors and delta opioid receptors were more efficiently returned to plasma membrane after the remifentanil-induced internalization.

用瑞芬太尼处理转染金星融合的MU阿片受体和蓝宝石融合的Delta阿片受体的幼年仓鼠肾细胞。为研究瑞芬太尼对异源二聚体的内化和再增敏作用，采用激光共聚焦显微镜观察瑞芬太尼对细胞的内化和再增敏作用。荧光共振能量转移分析证实了Mu阿片受体和Delta阿片受体的异二聚化。瑞芬太尼以剂量依赖的方式引起Mu-Delta异二聚体内化。与我们之前使用单独转Mu阿片受体的新生仓鼠肾细胞的研究相比，转Mu阿片受体和Delta阿片受体的幼鼠肾细胞在瑞芬太尼诱导的内化后更有效地返回质膜。

项目成果

GABA_B receptors do not internalize after baclofen treatment possibly due to lack of theβ-arrestin association ; a real-time visualization study

巴氯芬治疗后 GABA_B 受体不会内化，可能是由于缺乏实时可视化研究；

• DOI: 10.1002/syn.21565
• 发表时间: 2012
• 期刊: Synapse, Epub ahead of print
• 作者: Sudo;Y.;Hojo;M.;Ando;Y.;Takada;M.;Murata;H.;Kurata;S.;Kanaide;M.;Nishida;N.;Uezono;Y.
• 通讯作者: Y.

GABAB receptors do not internalize after baclofen treatment, possibly due to a lack of ss-arrestin association : study with a real-time visualizing assay

GABAB 受体在巴氯芬治疗后不会内化，可能是由于缺乏 ss-arrestin 关联：实时可视化分析研究

• 期刊: Synapse
• 影响因子: 2.3
• 作者: 須藤結香、村田寛明、上園保仁;他
• 通讯作者: 他