Multilateral study of prestin expression and function in mouse choclear outer hair cell

小鼠胆透明外毛细胞中prestin表达和功能的多边研究

• 批准号: 22791562
• 负责人: ABE Takahisa
• 金额: $ 2.58万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791562/
• 关键词: マウス; 外有毛細胞; プレスチン; 発達; 運動能; prestin; 発達変化; Gjb2

Voltage-dependent, nonlinear capacitance (Cv), which represents the conformational fluctuations of the motor molecule, progressively increased during development while Clin, which represents the membrane area of OHC, remained a relatively small increase with development. The time course of Cv and Clin in R75W transgenic mice and non-transgenic mice showed no significant difference, further showing that they were almost identical to that in C57BL/6J mice (Abe et al., 2007). Morphological study using digitally captured phase-contrast images of developing OHCs showed no significant difference between R75W transgenic mice and C57BL/6J mice. Our results indicate that development of OHC motility function is not affected in R75W transgenic mice.

电压依赖性，非线性电容（Cv），这代表的构象波动的电机分子，在发展过程中逐步增加，而临床，这代表OHC的膜面积，保持一个相对较小的增加与发展。R75 W转基因小鼠和非转基因小鼠中Cv和Clin的时间进程没有显示出显著差异，进一步表明它们与C57 BL/6 J小鼠中的Cv和Clin的时间进程几乎相同（Abe等人，2007年）。使用数字捕获的相位衬度图像的形态学研究显示，R75 W转基因小鼠和C57 BL/6 J小鼠之间没有显着差异。我们的研究结果表明，OHC运动功能的发展是不受影响的R75 W转基因小鼠。