Microbicidal activities of low frequency atmospheric pressure plasma jets on root canal treatment

使用低频大气压等离子体射流(LF JET)的研究正变得越来越普遍。我们进行了实验，以评估该技术对口腔病原微生物的杀菌效果，并确定其临床应用的潜力。我们进行了固体表面的直接暴露试验、液体的间接暴露试验和ROS(活性氧物种)抑制试验。结果表明，LF射流对口腔病原菌有杀灭作用，ROS对杀菌效果有影响。本研究的实验表明，LF JET对固、液两种形态的口腔病原菌均有杀菌作用。其杀菌机理可能与超氧阴离子自由基作用有关。这些结果表明，低频喷射技术可能是一种可应用于临床牙科领域的新技术。

Research using low frequency atmospheric pressure plasma jets(LF jet) is becoming increasingly more common. We carried out experiments to evaluate the sterilizing effects of this technology on oral pathogenic microorganisms and to determine its potential for clinical application. We performed the direct exposure test on a solid surface, indirect exposure test on a liquid phase, and ROS(reactive oxygen species) inhibitory test. The results showed the LF jet had microbicidal effects on oral pathogens, and that the ROS influenced this sterilization effect. The experiments of this study revealed that LF jet had a sterilizing effect on oral pathogenic microorganisms present in both the solid and liquid phases. The sterilizing mechanism was considered to be related to the effect of superoxide anion radicals. These results indicate that LF jets may represent a novel technology that can be applied to the field of clinical dentistry.