Microbicidal activities of low frequency atmospheric pressure plasma jets on root canal treatment

利用低频大气压等离子体射流（LF jet）的研究越来越普遍。我们通过实验来评价该技术对口腔病原微生物的灭菌效果，并确定其临床应用潜力。我们在固体表面进行了直接暴露试验，在液相进行了间接暴露试验，并进行了ROS（活性氧）抑制试验。结果表明，LF射流对口腔病原菌具有一定的杀灭作用，而活性氧影响了这种杀灭作用。本研究的实验表明，LF射流对口腔内固相和液相的病原菌均有一定的杀菌作用。认为其杀菌机理与超氧阴离子自由基的作用有关。这些结果表明，LF射流可能是一种可以应用于临床牙科领域的新技术。

Research using low frequency atmospheric pressure plasma jets(LF jet) is becoming increasingly more common. We carried out experiments to evaluate the sterilizing effects of this technology on oral pathogenic microorganisms and to determine its potential for clinical application. We performed the direct exposure test on a solid surface, indirect exposure test on a liquid phase, and ROS(reactive oxygen species) inhibitory test. The results showed the LF jet had microbicidal effects on oral pathogens, and that the ROS influenced this sterilization effect. The experiments of this study revealed that LF jet had a sterilizing effect on oral pathogenic microorganisms present in both the solid and liquid phases. The sterilizing mechanism was considered to be related to the effect of superoxide anion radicals. These results indicate that LF jets may represent a novel technology that can be applied to the field of clinical dentistry.