Study of molecular mechanisms of salivary gland development by Snail family genes.

蜗牛家族基因唾液腺发育的分子机制研究。

• 批准号: 22791973
• 负责人: TANIGUCHI Yoshitaka
• 金额: $ 2.58万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791973/
• 关键词: 唾液腺発達; Snail; 唾液腺; 発達; 分枝発達; Snailファミリー; Branching; Snaiファミリー

Snail family genes, snail and slug, which were identified as a epithelial mesenchymal transition, were expressed in epithelial cells of developing salivary glands. We examined the effect of gene-knock down and over-expression of snail family genes on development of salivary gland in explant culture. We found that branching development was inhibited by Snail/ Slug siRNA treatment and stimulated by Snail/ Slug over-expression. These data suggested that Snail and Slug plays stimulatory roles in branching development of salivary gland.

蜗牛家族基因Snail和Slug被鉴定为上皮间充质转化基因，在发育中的唾液腺上皮细胞中表达。在组织培养中，我们研究了蜗牛家族基因的敲除和过表达对唾液腺发育的影响。我们发现，Snail/Slug siRNA处理抑制了分枝发育，而Snail/Slug过表达则促进了分枝发育。这些数据表明，蜗牛和鼻涕在唾液腺的分枝发育中起着促进作用。