Physiological significance of phosphatidylinositol 3-kinase complexes

ATG6/VPS30是酵母磷脂酰肌醇3-激酶(PI3-Kinase)复合体的组成部分，参与自噬和空泡蛋白分选(VPS)途径。然而，在高等真核生物中，ATG6/VPS30同系物在囊泡运输中的意义一直存在争议。在这项研究中，我创造了atatg6纯合拟南芥植株，这些植株表现出严重的生长缺陷。在这个突变体中，PI3激酶的活性似乎严重降低。基因芯片分析表明，在atatg6突变体中诱导了大量的基因。该突变体可作为进一步研究植物PI3K复合体新的生理功能的模型系统。

ATG6/VPS30, which is a component of yeast phosphatidylinositol 3-kinase(PI3-kinase)complexes, is involved in autophagy and vacuolar protein sorting(VPS)pathway. In higher eukaryotes, however, significance of ATG6/VPS30 homologues in vesicle trafficking has been in dispute. In this study, I created atatg6 homozygous Arabidopsis plants, which exhibited severe growth defects. PI3 kinase activity seems to be severely reduced in this mutant. Microarray analyses revealed that numerous genes are induced in atatg6 mutant. This mutant should serve as a model system for further investigating novel physiological functions of plant PI3K complexes.