Identification of the gene coding tyrosine hydroxylase using the transposon-tagging methods.

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The genomic region including promoter of the DOPA dioxygense(DOD) gene was isolated from Mirabilis jalapa. DNA sequence analysis revealed that the DOD gene was consisted of 3 exons and 2 introns. We, however, could not find any transposable element-like sequences in the genomic region. Three candidate cDNAs encoding tyrosine hydroxylase were isolated from petals of Mirabilis jalapa using cDNA subtraction method.