栄養素の過不足を利用したプリ骨格異常モデルの構築

在孵化后第2天(DPH)和第14天(DPH)，Calcein荧光染色成功地显示了黄尾鱼的脊索、齿状突起、鳃弓和脑颅。钙黄绿素染色还显示了15DPH左右的神经脊椎和其他背部骨骼元素。然而，在18DPH左右，鱼体表面形成的色素阻碍了对鱼骨的直接观察。此外，在约22DPH时，鱼体的增厚通常妨碍直接观察到鱼的中轴线骨骼，而在27DPH时则变得不可能。为了分析生长抑制与骨骼畸形发生的一致性，对同一批次不同大小的鱼进行了骨骼畸形的发生情况的检测。在1^&lt；st&gt；实验中，观察到体型越大的鱼，骨骼缺陷的发生率就越高。然而，明显相反的是，体型较小的鱼显示出更高的骨骼畸形发生率。因此，鱼类的生长速度与骨骼畸形的发生之间没有明显的关系。

Fluorescent calcein staining successfully visualized cleithrum, and dentary, gill arch and neurocranium of yellowtail at 2 and 14 days post hatching(dph), respectively. Calcein staining also visualized neural spine and other elements of back bone around 15 dph. However, pigment formation at the surface of fish body hinder direct observation of back bone of fish at around 18 dph. Moreover, thickening of body often prevented direct observation of axial skeleton in fish at around 22 dph and becomes impossible at 27 dph. To analyze coincidence of growth depression and occurrence of skeletal deformity, occurrence of bone deformity was examined in fish showing different sizes from same batch of fish. in the 1^<st> experiment, significantly higher incidence of skeletal defomrity was observed in fish from bigger size of fish. However, in apparent contrat, smaller size fish showed higher frequency of occurrence of skeletal deformity. Therefore, there was no clear relationship between growth rate and occurrence of skeletal deformity in fish.