栄養素の過不足を利用したプリ骨格異常モデルの構築

利用营养过剩或缺乏构建骨骼异常前模型

• 批准号: 22780192
• 负责人: HAGA Yutaka
• 金额: $ 2.58万
• 依托单位: Tokyo University of Marine Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780192/
• 关键词: 水族栄養; ブリ; 骨格異常; モデル; ビタミン

Fluorescent calcein staining successfully visualized cleithrum, and dentary, gill arch and neurocranium of yellowtail at 2 and 14 days post hatching(dph), respectively. Calcein staining also visualized neural spine and other elements of back bone around 15 dph. However, pigment formation at the surface of fish body hinder direct observation of back bone of fish at around 18 dph. Moreover, thickening of body often prevented direct observation of axial skeleton in fish at around 22 dph and becomes impossible at 27 dph. To analyze coincidence of growth depression and occurrence of skeletal deformity, occurrence of bone deformity was examined in fish showing different sizes from same batch of fish. in the 1^<st> experiment, significantly higher incidence of skeletal defomrity was observed in fish from bigger size of fish. However, in apparent contrat, smaller size fish showed higher frequency of occurrence of skeletal deformity. Therefore, there was no clear relationship between growth rate and occurrence of skeletal deformity in fish.

荧光钙黄绿素染色成功地显示了黄尾鱼在孵化后2天和14天的舌骨、齿骨、鳃弓和脑颅。钙黄绿素染色还显示了神经棘和脊椎骨的其他元素约15 dph。然而，鱼体表面的色素形成阻碍了在18 dph左右直接观察鱼的脊骨。此外，身体变厚往往阻碍了在22 dph左右直接观察鱼类的中轴骨骼，在27 dph时变得不可能。为了分析生长抑制与骨骼畸形发生的一致性，对来自同一批鱼的不同大小的鱼进行了骨骼畸形发生的检查。在第1次实验中<st>，在来自较大尺寸的鱼的鱼中观察到显著更高的骨骼畸形发生率。然而，在明显的对比中，较小的鱼显示出更高的骨骼畸形发生率。因此，生长速度和鱼类骨骼畸形的发生之间没有明确的关系。