Development of environmentally friendly pretreatment using ferric catalyst as abrasive for enzymatic hydrolysis of lignocellulosic biomass

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To achieve highly effective enzymatic hydrolysis of Japanese cypress (Chamaecyparis obtusa), softwood, the combination of dried disk milling with abrasives and alkaline-peroxide treatment was investigated, and their suitable conditions were decided. The treated sample had highly enzymatic digestibility because this combination caused the degradation of almost of hemicellulose and 60% of lignin, and the exposing cellulose nanofiber and nano scale " raising" on the surface. Moreover, the suitable enzyme was decided, and it was possible to reduce the amount of loading enzymes.