Development of Noble Structure PI Polyamide Recognizing to a Unique Sequence on double-strand DNA

具有0-丙氨酸/Py或β-丙氨酸/Im的交叉型PI聚酰胺成功地制成了靶全DNA序列，而没有GGGG和CCCC。然后，为了合成识别12个碱基对DNA序列的长链PI聚酰胺，开发了使用HMPB树脂通过固相合成2-3 mer PI聚酰胺嵌段，并且可以合成延长这些PI聚酰胺嵌段的长链PI聚酰胺。固相合成的环状PI聚酰胺比发夹型PI聚酰胺具有更强的DNA结合能力。

Cross form PI polyamide having 0alaninelPy or p-alanine/lm was succeeded in making into a target all DNA sequence without GGGG and CCCC. Then, for the synthesis of long chain PI polyamide recognizing 12 base-pair DNA sequence, 2-3 mer PI polyamide blocks by solid phase synthesis using HMPB resin was developed and the long chain PI polyamide could be synthesized elongating these PI polyamide blocks. And it was established that solid phase synthesis of the cyclic PI polyamides more strong DNA binding than hair-pin PI polyamide.