Individual identification by discrimination of mismatch nucleotides using RNase A
Individual identification by discrimination of mismatch nucleotides using RNase A
批准号:
22790592
负责人:
ASARI Masaru
金额:
$2.41万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
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英文摘要
In 261G/deletion and 796C/A on the human ABO gene, we could genotype the SNP alleles based on the mismatches between template-probe duplexes using synthesized RNA, DNA/RNA chimeric probes, and RNase A. The lengths of probes and the positions of RNA bases corresponding to the SNP affected the sensitivity and specificity of genotyping. Furthermore, the genotypes in two loci were identified by probes designed with the different sizes.
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会议论文
21座位STR判定に基づく身元確認のためのDNA鑑定
基于 21 位点 STR 测定的 DNA 测试以确认身份
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[浅利優, 大村友博, 岡久美子, 山田ひろみ, 土井大輝, 間瀬田千香暁, 塩野寛, 松原和夫, 松田光悦, 清水惠子]
通讯作者:
清水惠子
Multiplex PCR-based Alu insertion polymorphisms genotyping for identifying individuals of Japanese ethnicity
基于多重 PCR 的 Alu 插入多态性基因分型用于识别日本种族个体
DOI:
10.1016/j.ygeno.2012.01.004
发表时间:
2012
期刊:
Genomics
影响因子:
4.4
作者:
[M. Asari, T. Omura, K. Oka, C. Maseda, Y. Tasaki, H. Shiono, K. Matsubara, M. Matsuda, K. Shimizu]
通讯作者:
K. Shimizu
Genotyping of insertion/deletion polymorphisms using locked nucleic acids for human identification
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批准号:24590847
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.49万
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财政年份:2012
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负责人:ASARI Masaru
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依托单位:
海外基金