Comprehensive study of hepatitis B virus genotypes and mutations involved in the pathogenesis

乙型肝炎病毒基因型及突变参与发病机制的综合研究

• 批准号: 22790627
• 负责人: INOUE Jun
• 金额: $ 2.33万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790627/
• 关键词: 肝臓学; ウイルス性肝炎

To investigate hepatitis B virus (HBV)genotypes and mutations that are involved in the pathogenesis, full-genome analysis of genotype B1/Bj HBV, which is known to occur fulminant hepatitis frequently, was performed. Interestingly, single nucleotide insertion/deletion that causes frameshift in the precore region was found frequently in fulminant hepatitis strains. Furthermore, amino acid 21 mutation in the core protein was also found frequently. In vitro studies clarified that HBV with these insertion/deletion/mutation showed high replication capacity. These findings might clarify a part of fulminant hepatitis pathogenesis.

为探讨乙肝病毒基因分型和变异与发病机制的关系，对常见的重型肝炎病毒B1/Bj型进行了全基因组分析。有趣的是，导致前核心区移码的单核苷酸插入/缺失在暴发型肝炎病毒株中经常被发现。此外，核心蛋白的21位氨基酸突变也很常见。体外研究表明，具有这些插入/缺失/突变的乙肝病毒具有很高的复制能力。这些发现可能在一定程度上阐明重型肝炎的发病机制。

项目成果

Management of chronic hepatitis B patients : Efficacy & limitation of nucleos(t)ide analogues

慢性乙型肝炎患者的管理：疗效

• 发表时间: 2011
• 期刊: Indian Journal of Medical Research
• 影响因子: 4.2
• 作者: Inoue J;Ueno Y;Shimosegawa T
• 通讯作者: Shimosegawa T

Inhibitory effect on hepatitis B virus in vitro by a peroxisome proliferator-activated receptor-γ ligand, rosiglitazone

过氧化物酶体增殖物激活受体-γ配体罗格列酮对乙型肝炎病毒的体外抑制作用

• 发表时间: 2010
• 期刊: Biochemical and Biophysical Research Communications
• 影响因子: 3.1
• 作者: Wakui Y;Inoue J;Ueno Y;Fukushima K;Kondo Y;Kakazu E;Obara N;Kimura O;Shimosegawa T
• 通讯作者: Shimosegawa T

B型劇症肝炎におけるgenotype, precore領域frameshiftの意義

暴发性乙型肝炎基因型和前核心区移码的意义

• 发表时间: 2011
• 期刊: 消化器内科
• 作者: 井上淳;上野義之;下瀬川徹
• 通讯作者: 下瀬川徹

Yasuteru Kondo, Eiji Kakazu, Masashi Ninomiya, Osamu Kimura, Tooru Shimosegawa. Association between Mutations in a Cytotoxic T Lymphocyte Epitope of Hepatitis B Virus and Fulminant Hepatitis

近藤康辉、加一英二、二宫正志、木村修、下濑川彻。

• 发表时间: 2011
• 作者: Jun Inoue;Yoshiyuki Ueno;Hirofumi Niitsuma
• 通讯作者: Hirofumi Niitsuma

The Significance of Frameshift in the Precore Region of Hepatitis B Virus Obtained from Fulminant Hepatitis Patients

暴发性肝炎患者乙型肝炎病毒前核心区移码的意义

• 发表时间: 2010
• 作者: Jun Inoue;Yoshiyuki Ueno;Yuta Wakui;Koji Fukushima;Yasuteru Kondo;Eiji Kakazu;Masashi Ninomiya;Hirofumi Niitsuma;Tooru Shimosegawa
• 通讯作者: Tooru Shimosegawa