A new regulative mechanism for monoamine transporter expression in plasma membrane : The participation of histamine H3 receptor

为了研究组胺H3受体(H3R)参与单胺转运体向质膜的转化，以发挥其转运体的功能，我们利用H3R和Net共表达细胞系统研究了H3R与去甲肾上腺素转运体(NET)表达的关系。本实验结果表明，H3R通过与Net结合，使Net与细胞紧密结合，从而抑制Net进入质膜的表达，从而抑制Net的表达。

To study the participation of histamine H3 receptor (H3R) on the transition of monoamine transporters into plasma membrane which is necessary to exert their function as transporters, we investigated the relation between H3R and noradrenaline transporter (NET) expression by using coexpressing cell system for both H3R and NET. The results which obtained from this experiment were suggested that there was a mechanism by which H3R inhibited the NET expression into the plasma membrane by binding to NET and making it fit tightly in the cell.