Analyses of molecular mechanisms of salivary secretion in salivary acinar cells using gene expression in vivo

通过逆行导管注射腺病毒载体在大鼠下颌下腺中表达Stim 1-mKO 1。Stim 1-mKO 1蛋白主要在腺病毒载体感染后的腺泡细胞中表达。共聚焦显微镜和Ca ~（2+）成像分析表明，下颌下腺腺泡细胞中表达的Stim 1-mKO 1是功能性的。Stim 1-mKO 1的过表达增强激动剂诱导的细胞内钙库的钙释放和钙库操作的钙内流。与对照组相比，Stim 1的过表达可能增加了唾液分泌。此外，我们成功地监测了激动剂诱导的钙离子动力学在SMG在活的动物使用钙离子指示剂，G-GECO。

Stim1-mKO1 was expressed in rat submandibular glands by retrograde ductal injection of an adenoviral vector. Stim1-mKO1 proteins were expressed predominantly in acinar cells following infection with an adenoviral vector. Confocal microscopy and Ca^ imaging analysis indicated that the Stim1-mKO1 expressed in submandibular acinar cells is functional. The overexpression of Stim1-mKO1 enhanced the agonist-induced Ca^ release from intracellular Ca^ stores and the Ca^ entry through store-operated Ca^ entry. It is possible that the overexpression of Stim1 increased salivary fluid secretion compared to the control. In addition, we succeeded in monitoring the agonist-induced Ca^ dynamics in SMG in live animals using the Ca^ indicator, G-GECO.