Study on the regulation mechanism of oxidative stress for treatment of dry mouth in Sjogren's syndrome

氧化应激治疗干燥综合征口干的调节机制研究

• 批准号: 22592085
• 负责人: SOHDA Miwa
• 金额: $ 2.83万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592085/
• 关键词: 歯学; 細胞組織; 酸化ストレス; 唾液腺; 組織・細胞; 細胞・組織; ゴルジ体; コイルドコイル構造

Sjogren’s syndrome is a chronic inflammatory autoimmune disorder that affects mainly salivary and lacrimal gland, resulting in dry mouth and dry eyes symptoms. In this study, to understand the regulatory mechanism of inflammation in salivary gland, oxidative stress response was investigated using human submaxillary salivary gland A-253 cells. A whole human genome micro-array analysis showed that the expression levels of pro-inflammatory cytokines such as IL-6, IL-18 and CXCL1 were increased in H2O2 treated cells compared with control cells. While, anti-inflammatory cytokine IL-11 was up-regulated in H2O2 treated cells. These results suggest that the target tissue itself might have a role on regulation of peripheral inflammation. IL-11 might be a candidate molecule for substitution therapy. Furthermore, a soluble isoform of IL-17 receptor A (IL-17RA) was identified in human salivary gland. IL-17 was supposed to a key molecule of inflammatory disorders such as Sjogren’s syndrome, rheumatoid arthritis and Crohn’s disease. The soluble isoform of IL-17 RA could be an endogenous antagonist against the IL-17 signaling.

Sjogren综合征是一种慢性炎症性自身免疫性疾病，主要影响唾液和泪腺，导致口干和眼睛干燥的符号。在这项研究中，为了了解唾液腺中感染的调节机制，使用人类颌面唾液腺A-253细胞研究了氧化应激反应。整个人类基因组微阵列分析表明，与对照细胞相比，H2O2处理的细胞中促炎性细胞因子（例如IL-6，IL-18和CXCL1）的表达水平增加。同时，在H2O2处理的细胞中，抗​​炎细胞因子IL-11上调。这些结果表明，靶组织本身可能在调节外周感染中起作用。 IL-11可能是用于取代治疗的候选分子。此外，在人类唾液腺中鉴定出IL-17受体A（IL-17RA）的固体同工型。 IL-17被认为是炎性疾病的关键分子，例如Sjogren综合征，类风湿关节炎和克罗恩病。 IL-17 RA的可溶性同工型可能是对IL-17信号传导的内源性拮抗剂。

项目成果

Interaction of Golgin-84 with the COG Complex Mediates the Intra-Golgi Retrograde Transport

• DOI: 10.1111/j.1600-0854.2010.01123.x
• 发表时间: 2010-12-01
• 期刊: TRAFFIC
• 影响因子: 4.5
• 作者: Sohda, Miwa;Misumi, Yoshio;Oda, Kimimitsu
• 通讯作者: Oda, Kimimitsu

Interaction of Golgin-84 with the conserved oligomeric Golgi (COG) complex mediates the intra-Golgi retrograde transport

Golgin-84 与保守寡聚高尔基体 (COG) 复合体的相互作用介导高尔基体内逆行运输

• 发表时间: 2010
• 作者: Sohda M;Misumi Y;Yamamoto A;Nakamura N;Oda K
• 通讯作者: Oda K

Interaction of Golgin-84 with the conserved oligomeric Golgi(COG)complex mediates the intra-Golgi retrograde transport

Golgin-84 与保守寡聚高尔基体 (COG) 复合物的相互作用介导高尔基体内逆行运输

• 发表时间: 2010
• 作者: Sohda M;Misumi Y;Yamamoto A;Nakamura N;Oda K
• 通讯作者: Oda K

Golgins and the COG complex

戈尔金斯和 COG 复合体

• 发表时间: 2011
• 作者: Sohda M;Nakamura N;Misumi Y
• 通讯作者: Misumi Y