Development of adhesion system for one-step step bonding agent

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To determine the amount of 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-calcium (MDP-Ca) salt produced through demineralization of enamel or dentin by MDP, we designed a series of MDP-based one-step adhesives with different amounts of MDP. The null hypotheses were that (1) the amount of MDP-Ca salt produced through demineralization of enamel was the same as dentin, and (2) the amounts of MDP-Ca salt have no effect on the bonding performance. Increases in the amount of MDP resulted in the increased amount of MDP-Ca salt. The production amount of MDP-Ca salt of the dentin was 1.3 times higher than that of the enamel. The predominate species of the MDP and enamel reactants was a calcium hydrogen phosphate of MDP. In contrast, the dentin yielded both calcium phosphate and calcium hydrogen phosphate of MDP. Increases in the amount of MDP-Ca salt decreased both enamel and dentin bond strengths. An optimal concentration of MDP exists in one-step self-etch adhesive.