Development of Adhesive Primer to Dentin

以甲基丙烯酰氯为原料，在氢氧化钠存在下，将N甲基丙烯酰氯缩合成相应于欧米伽氨基酸的氨基，合成了5种NMomegaA，NMGIy(甘氨酸)、NMbetaAla(β-Valanine)、NMBU(γ-氨基丁酸)、NMVa(β-氨基戊酸)和NMCA-氨基己酸(NMCA)。研究了底漆与脱矿牙本质的相互作用强度对树脂与酸蚀牙本质粘结强度的影响。当牙本质胶原蛋白分散到NMomegaA溶液中时，NMomegaA分子中两个由酰胺和羧酸组成的羰基碳的T1值显著降低。这一结果是由于NMomegaA分子中的酰胺基团和羧酸基与牙本质胶原分子之间的相互作用所致。随着NMomegaA分子中亚甲基数目的增加，这些羰基碳的T_1值减小。随着NMomegaA分子中亚甲基数目的增加，相互作用变得更强。此外，树脂与以NMomegaA为底漆的酸蚀牙本质的粘结强度随着酰胺基碳的T_1值的降低而增加。NMomegaA底漆与牙本质胶原分子的相互作用强度通过核磁共振技术测定，显示与NMomegaA底漆处理过的酸蚀牙本质上的树脂粘结强度直接相关。用~(113)C NMomegaR技术研究几种底物在脱矿牙本质上的吸附行为。因为NMomegaR技术对研究几种类型的相互作用非常敏感。

Five species of NMomegaA, NMGIy (glycine), NMbetaAla (beta-Valanine), NMBu (gamma-amino butyric acid), NMVa deltaamino valeric acid) and NMCa epsilon-amino caproic acid) were synthesized with the condensation of methacryloyl chloride to the amino group which corresponded to the omega-amino acid in the presence of NaOH.The interaction between NMomegaA primers and dentinal collagen were studied using the ^<13>C NMR technique including the observation of spin-lattice relaxation times, T_1. The effect of the strength of the interaction of the primer to the demineralized dentin on the bond strength of resin to the acid-etched dentin was examined.When the dentinal collagen was dispersed into the NMomegaA solution, the T_1 values of the two carbonyl carbons attributed to the amide and the carboxylic acid in the NMomegaA molecule decreased dramatically. This result was due to the interaction between the amide group and the carboxylic acid group in the NMomegaA molecule and the dentinal collagen molecule. The T_1 value of these carbonyl carbons decreased when the number of methylene in the NMomegaA molecule increased. The interaction became stronger, as the number of methylene in the NMomegaA molecule was increased. Further, the bond strength of the resin to the acid-etched dentin primed with NMomegaA increased with a decrease in the T_1 value of the amide carbonyl carbon. The strength of the interaction of the NMomegaA primer to the dentinal collagen molecule determined by ^<13>C NMR technique showed a direct correlation with the bond strength of the resin to acid-etched dentin that was treated with the NMomegaA primer. The adsorption behavior of the several types of primers onto the demineralized dentin will be discussed by using ^<113>C NMomegaR technique. Because the NMomegaR technique is very sensitive to investigate the several types of interaction.