Development of Adhesive Primer to Dentin

在NaOH存在下，甲基丙烯酰氯与ω -氨基酸对应的氨基缩合，合成了NMomegaA、NMGIy（甘氨酸）、NMbetaAla （β -缬氨酸）、NMBu （γ -氨基丁酸）、NMVa -三角氨基戊酸和NMCa - ε -氨基己酸5种化合物。采用^<13>C核磁共振技术研究了NMomegaA引物与牙本质胶原的相互作用，包括观察自旋晶格弛豫时间T_1。考察了底漆与脱矿牙本质相互作用强度对树脂与酸蚀牙本质结合强度的影响。将牙本质胶原分散到NMomegaA溶液中，NMomegaA分子中归属于酰胺和羧酸的两个羰基碳的T_1值显著降低。这是由于NMomegaA分子中的酰胺基团和羧酸基团与牙本质胶原分子相互作用的结果。这些羰基碳的T_1值随着NMomegaA分子中亚甲基数量的增加而降低。随着NMomegaA分子中亚甲基数量的增加，相互作用变得更强。此外，树脂与酸蚀牙本质的结合强度随着酰胺羰基碳T_1值的降低而增加。NMomegaA引物与牙本质胶原分子的相互作用强度通过^<13 b> C核磁共振技术测定，结果表明，NMomegaA引物与酸蚀牙本质的结合强度与树脂的结合强度直接相关。采用^<113>C NMomegaR技术，讨论了几种引物在脱矿牙本质上的吸附行为。因为NMomegaR技术对于研究几种类型的相互作用非常敏感。

Five species of NMomegaA, NMGIy (glycine), NMbetaAla (beta-Valanine), NMBu (gamma-amino butyric acid), NMVa deltaamino valeric acid) and NMCa epsilon-amino caproic acid) were synthesized with the condensation of methacryloyl chloride to the amino group which corresponded to the omega-amino acid in the presence of NaOH.The interaction between NMomegaA primers and dentinal collagen were studied using the ^<13>C NMR technique including the observation of spin-lattice relaxation times, T_1. The effect of the strength of the interaction of the primer to the demineralized dentin on the bond strength of resin to the acid-etched dentin was examined.When the dentinal collagen was dispersed into the NMomegaA solution, the T_1 values of the two carbonyl carbons attributed to the amide and the carboxylic acid in the NMomegaA molecule decreased dramatically. This result was due to the interaction between the amide group and the carboxylic acid group in the NMomegaA molecule and the dentinal collagen molecule. The T_1 value of these carbonyl carbons decreased when the number of methylene in the NMomegaA molecule increased. The interaction became stronger, as the number of methylene in the NMomegaA molecule was increased. Further, the bond strength of the resin to the acid-etched dentin primed with NMomegaA increased with a decrease in the T_1 value of the amide carbonyl carbon. The strength of the interaction of the NMomegaA primer to the dentinal collagen molecule determined by ^<13>C NMR technique showed a direct correlation with the bond strength of the resin to acid-etched dentin that was treated with the NMomegaA primer. The adsorption behavior of the several types of primers onto the demineralized dentin will be discussed by using ^<113>C NMomegaR technique. Because the NMomegaR technique is very sensitive to investigate the several types of interaction.