The analysis of histone modification in 4NQO-induced rat tongue cancer model

4NQO诱导的大鼠舌癌模型中组蛋白修饰分析

• 批准号: 22592211
• 负责人: YONEMOTO Kazuhiro
• 金额: $ 2.5万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592211/
• 关键词: 口腔癌; 癌; 遺伝子; 動物

We aim to examine the usefulness of 4-NQO induced rat tongue cancer as human oral carcinogenesis model .In order to verify whether changes in the overall level of H3K4,H3K9 histone methylation,the level of H3K4me3,H3K9me2,me3 were measured in 4-NQO induced rat tongue cancer model by western blotting.Our results suggest that the level of H3K9me2 was increased in squamous cell carcinoma of rat at 32 weeks from beginning of administration.Therefore, 4-NQO induced rat tongue cancer model is suggested the usefulness as human oral carcinogenesis model .

我们的目的是检验4-NQO诱导的大鼠舌癌作为人类口腔癌发生模型的有用性。为了验证H3 K4、H3 K9组蛋白甲基化的总体水平、H3 K4 me 3、H3 K9 me 2、me 3在4-结果表明，NQO诱导的大鼠舌癌模型中H3 K9 me 2蛋白表达水平在32 ℃时明显升高因此，提示4-NQO诱导的大鼠舌癌模型可用作人口腔癌发生模型。