Development of a highly sensitive genetic diagnosis ofSjogren syndrome based on detection ofβdefensins

为了解干燥综合征患者唇腺β防御素基因表达变化的细节，本研究对干燥综合征患者和非干燥综合征患者唇腺组织中β防御素S的免疫反应性和基因表达进行了研究。此外，我们还分析了β防御素在患病建筑小鼠模型小鼠气管上皮中的定位模式。这项研究的结果表明，急性炎症增加了β防御素4，慢性炎症降低了β防御素4。人β-Defensin4基因在干燥综合征患者的唇腺中表达较弱，而在非干燥综合征患者的唇腺中几乎不表达。有趣的是，没有干燥综合征的唇腺亚群显示出人类β-防御素4的基因表达，并且形态与干燥综合征相似。这些发现以及急性炎症增加β防御素4和慢性炎症降低β防御素4的结果可能预示着未来发生干燥综合征的可能性。这些分析可能被证明对干燥综合征的诊断和风险评估有用。

To analyze the details of changes in expression patterns of human β-defensins in labial glands in Sjogren’s syndrome, the present study investigated the immunoreactivity and gene expression of human β-defensin s in patients with and without Sjogren’s syndrome. Otherwise, we analyzed the localization patterns of β defensins , in the tracheal epithelium of mouse modeles of sick building mice. The results of this study indicated that acute inflammation increased β defensin4 and chronic inflammation decreased β defensin4. Weak gene expression of human β-defensin4 was detected in labial glands with Sjogren’s syndrome, but was almost absent in labial glands without Sjogren’s syndrome. Interestingly, a subgroup of labial glands without Sjogren’s syndrome showed gene expression of human β-defensin4and similar morphology to the Sjogren’s syndrome. These findings and the result that acute inflammation increased β defensin4 and chronic inflammation decreased β defensin4 may indicate a potential for future Sjogren’s syndrome. These analyses might prove useful in the diagnosing and assessing risk of Sjogren’s syndrome.