The analysis of the graft from the viewpoint with the relation between malignancy of oral carcinoma and defensin

确定人-防御素-2和人-防御素在牙源性角化囊肿和根性囊肿中的位置。人β -防御素-2免疫反应性在牙源性角化囊肿（72%）和根状囊肿（37%）中很明显。在两种类型的囊肿的角化细胞和颗粒层的细胞质中检测到人β -防御素-2的信号。人β -防御素-2的定位在囊肿和正常粘膜之间没有差异。在两种类型的囊肿中均检测到人α -防御素的免疫反应性，但在角质形成细胞中未检测到，在固有层的中性粒细胞细胞质中检测到人α -防御素的信号。许多囊肿产生人β -防御素-2和人aloha防御素，这表明两种类型的囊肿以及正常口腔粘膜中存在相似的利用防御素的防御机制。下一步的研究是证明免疫组织化学定位和分布的人β -防御素，肽抗菌活性，在干燥综合征和口干组织。组织样品包埋石蜡，用链亲和素-生物素偶联过氧化物酶法免疫染色β -防御素。用抗-防御素抗体对口干症患者和对照组（非口干症和非干燥综合征）的唇腺进行染色。然而，干燥综合征患者的唇腺未被抗-防御素抗体染色。因此，β -防御素被认为是干燥综合征的重要因素。

To identify the location of human-beta-defensin-2 and human alpha-defensins in odontogenic keratocysts and radicular cysts. Human beta-defensin-2 immunoreactivity was evident in odontogenic keratocysts(72%), and in radicular cysts(37%). Signals for human beta-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localization of human beta-defensin-2 did not differ between cysts and normal mucosa. Human alpha-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes, Signals for human aloha-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Many cysts generate human beta-defensin-2 and human aloha-defensins suggesting that similar defense mechanisms utilizing defensins exist in both types of cysts, as well as in normal oral mucosa.Next study was to demonstrate the immunohistochemical localization and distribution of human beta-defensins, peptides with antimicrobial activity, in Sjogren's syndrome and Xerostomia tissues. Tissue samples were embedded in paraffin and beta-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Labial glands in Xerostomia patients and control patients(non- Xerostomia and non- Sjogren's syndrome) were stained with anti-beta-defensins antibody. However, labial glands in Sjogren's syndrome were not stained with anti-beta-defensins antibody.Therefore, beta-defensins have been suggested to be important factors in Sjogren's syndrome.