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The analysis of the graft from the viewpoint with the relation between malignancy of oral carcinoma and defensin

从口腔癌恶性程度与防御素关系角度分析移植物

基本信息

批准号：
16591998
负责人：
MIZUKAWA Nobuyoshi
金额：
$ 2.11万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591998/
关键词：
antimicrobial peptide defensin HBD HNP graft oral carcinoma 移植再生 HBD-2

项目摘要

To identify the location of human-beta-defensin-2 and human alpha-defensins in odontogenic keratocysts and radicular cysts. Human beta-defensin-2 immunoreactivity was evident in odontogenic keratocysts(72%), and in radicular cysts(37%). Signals for human beta-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localization of human beta-defensin-2 did not differ between cysts and normal mucosa. Human alpha-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes, Signals for human aloha-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Many cysts generate human beta-defensin-2 and human aloha-defensins suggesting that similar defense mechanisms utilizing defensins exist in both types of cysts, as well as in normal oral mucosa.Next study was to demonstrate the immunohistochemical localization and distribution of human beta-defensins, peptides with antimicrobial activity, in Sjogren's syndrome and Xerostomia tissues. Tissue samples were embedded in paraffin and beta-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Labial glands in Xerostomia patients and control patients(non- Xerostomia and non- Sjogren's syndrome) were stained with anti-beta-defensins antibody. However, labial glands in Sjogren's syndrome were not stained with anti-beta-defensins antibody.Therefore, beta-defensins have been suggested to be important factors in Sjogren's syndrome.

确定人β-防御素2和人α-防御素在牙源性角化囊肿和根尖囊肿中的定位。人β-防御素-2免疫反应性在牙源性角化囊肿（72%）和根尖囊肿（37%）中明显。人β-防御素-2的信号在两种类型的囊肿的角化不全和颗粒层的角质形成细胞的细胞质中检测到。人类β-防御素-2的定位在囊肿和正常粘膜之间没有差异。在两种类型的囊肿中均检测到人α-防御素免疫反应性，但在角质形成细胞中未检测到。在固有层中的嗜中性粒细胞的细胞质中检测到人α-防御素的信号。许多囊肿产生人类β-防御素-2和人类aloha-防御素，这表明利用防御素的类似防御机制存在于两种类型的囊肿中，以及在正常的口腔mucosa.Next研究是证明人类β-防御素的免疫组织化学定位和分布，具有抗菌活性的肽，在干燥综合征和口干症组织中。将组织样品包埋在石蜡中，并通过链霉亲和素-生物素偶联过氧化物酶法对β-防御素进行免疫染色。用抗β-防御素抗体对口干症患者和对照患者（非口干症和非干燥综合征）的唇腺进行染色。而干燥综合征患者的唇腺中未发现抗β-防御素抗体，因此β-防御素可能是干燥综合征的重要因素。

项目成果

期刊论文数量（30）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Immunolocation of vascular endothelial grouwth factor during heterotopic bone formation induced from grafted periosteum.

移植骨膜诱导异位骨形成过程中血管内皮生长因子的免疫定位。

DOI：
发表时间：
2004
期刊：
Annals of Plastic Surgery. 53
影响因子：
0
作者：
Ueno T.;Mizukawa N.;Sygahara T
通讯作者：
Sygahara T

Clinical Effects of Cevimeline Hydrochloride for the Treatment of Dry Mouth in 2 patients with Sjogren's Syndrome

盐酸西维美林治疗干燥综合征口干2例临床疗效

DOI：
发表时间：
2005
期刊：
Journal of hard tissue biology 14(2)
影响因子：
0
作者：
Cui Z.-G.;et al.;K.NAGATA et al.;Takeda Y;Yamachika E;Mizukawa N;山村功ら;Tsujigiwa H;Fukunaga J;Takagi S;Nagatsuka H;Takagi S;Nobuhisa T;Nobuyoshi Mizukawa
通讯作者：
Nobuyoshi Mizukawa

Expression of human α-and β-defensins in odontogenic keratocysts and radicular cysts.

人α-和β-防御素在牙源性角化囊肿和根囊肿中的表达。

DOI：
发表时间：
2004
期刊：
Asian J oral maxillofac Surg 16
影响因子：
0
作者：
Cui Z.-G.;et al.;K.NAGATA et al.;Takeda Y;Yamachika E;Mizukawa N;山村功ら;Tsujigiwa H;Fukunaga J;Takagi S;Nagatsuka H;Takagi S;Nobuhisa T;Nobuyoshi Mizukawa;Yamachika E;植野高章;Fukunaga J;Shin Takagi;Fukunaga J;Yoshimoto T
通讯作者：
Yoshimoto T

β-TCPを用いてインプラント周囲欠損骨の骨再生を行った1症例

使用β-TCP进行种植体周围骨再生的案例