The aim at efficiency of transplantation by regulation the defensin expression

口腔黏膜对外界刺激、机械创伤和微生物病原体的生物屏障由口腔上皮细胞和炎症细胞组成。防御素是一种阳离子抗菌肽，分为人α-防御素（HNP）和人β-防御素（HBD）两类。HBD-2由皮肤的角质形成细胞产生，已知细菌感染、脂多糖（LPS）、白细胞介素（IL）-1 α、IL-1 β和TNF-α强烈上调HBD-2的表达。有趣的是，HBD-2的表达不仅在与细菌感染相关的炎症病变中观察到，而且在其他非炎症病变如癌中也观察到。此外，HBD-2在体外可诱导正常表皮细胞系（NHEK）增殖死亡，这种细胞增殖死亡效应与低剂量辐射效应相似。这可能反过来有助于鳞状细胞癌细胞的增殖，因为HBD-2对鳞状细胞癌细胞的存活没有直接影响。我们认为HBD-2的上调可能具有与防御机制无关的副作用，其可导致邻近SCC的角质形成细胞的死亡，从而间接地帮助肿瘤细胞的增殖。我们报道了防御素诱导肥大细胞释放组胺。已知组胺可诱发炎症。高浓度的防御素可能对周围正常细胞造成损伤。本研究的目的是通过调控防御素的表达，促进移植物的愈合。

The biophiraxic barriers against external stimuli, mechanical trauma and microbiological pathogens consist of the oral epithelia and inflammatory cells. Defensins are cationic antimicrobial peptides, which are categorized into two types, human α-defensins (HNPs) and human β-defensins (HBDs). HBD-2, which is produced by the keratinocytes of the skin, is known to be strongly up-regulated by bacterial infections, lipopolysaccharides (LPS), interleukin (IL)-1 alpha, IL-1 beta and TNF-alpha.Interestingly, HBD-2 expression is observed not only in the inflamed lesions associated with bacterial infection but also in other non-inflamed lesions such as carcinomas. Furthermore, so-called proliferation death of normal epidermal cells line (NHEK) was shown to be induced by HBD-2 generated by a squamous cell carcinoma cell line in vitro.We identified that this effect of cellular proliferation death, is similar to the effect of low-dose irradiation on cells. This may in turn, help the multiplication of squamous cell carcinoma cells, because HBD-2 had no direct effect on the survival of squamous cell carcinoma cells. We suggested that up regulation of HBD-2 might have side effects, unrelated to defense mechanisms, which would lead to the death of keratinocytes adjacent to the SCC which indirectly assists in the multiplication of tumor cells.Also, defensin was up-regulated in host side by transplantation as a surgical injury. We reported that defensin induced Histamine release from Mast Cells. Histamine was known to induce inflammation. And a High concentration of defensin was suggested to cause injury to neighboring normal cells. The purpose of our study was to regulate the defensin expression and obtain a favorable healing of grafts.