The study detects the prognosis and metastatic markers of oral cancer by imaging mass spectrometry and develops the molecular pathology diagnostic procedure

在这项研究中，我们试图根据成像质谱分析(IMS)可视化的生物分子分布来清楚地区分边界。M/z 770和m/z 846处的两个信号分布在每个区域，并显示清晰的边界。串联质谱分析确定这些信号在癌区的m/z 770处为磷脂酰胆碱(PC)(16：0/16：1)，在间质区域的m/z 846处为PC(18：1/20：4)。综上所述，通过IMS分析，这些PC物种可以清楚地区分口腔鳞癌细胞的癌区域和间质区域，分子鉴定可以为阐明肿瘤侵袭机制提供重要信息。

In this study, we tried to clearly distinguish the border based on the biomolecule distributions visualized by imaging mass spectrometry (IMS) analysis. Two signals at m/z 770 and m/z 846 were distributed on each region and showed a clear border. A tandem mass spectrometry analysis identified these signals as phosphatidylcholine (PC) (16:0/16:1) at m/z 770 on the cancer region and PC (18:1/20:4) at m/z 846 on the stroma region. In conclusion, the cancer region and stromal region of OSCCs were clearly distinguished with the use of these PC species by IMS analysis and the mo lecular identification can provide important information to elucidate the mechanism in cancer invasion.