Role of cancer stem cells related molecules in oral squamous cell carcinoma

肿瘤干细胞相关分子在口腔鳞癌中的作用

基本信息

批准号：
22592246
负责人：
YAMAZAKI Hiroshi
金额：
$ 2.91万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Oral squamous cell carcinoma (OSCC) include cellular heterogeneity, as would be expected if they arose from dysregulated stem or progenitor cells as opposed to the simple clonal expansion of a mutated cell; however, stemness molecule expression levels and distribution remain unclear. To overcome this problem, stemness molecule expressions were determined in OSCC as well as their properties and prognosis. Two chromatin regulators, Bmi-1 and Hmga2, were identified in 12 tumors region and background pair cases of OSCC by microarray. Both molecules are known to promote stem cell self-renewal by negatively regulating the expressions of Ink4a and Arf tumor suppressors. The expression levels were analyzed in 38 pair cases of OSCC by Qrt-PCR and in 91 cases of the same stage of tongue SCC. Despite a similar target, Bmi-1 protein was expressed in an early cancerous region and HMGA2 protein was expressed in a more progressed region. Likewise, Bmi1-expressing tumor had no significance with regard to overall survival (P = 0.67), while HMGA2-expressing tumors had decreased overall survival (P = 0.05). Qrt-PCR analyses also correlated with protein levels. These findings suggest that Bmi-1 is an early detection marker to distinguish cancerous from non-cancerous regions, while HMGA2 is presumed to be a tumor prognosis marker. Among our OSCC analyses, these stemness molecules expressed not so many primitive rare cells in the tumor as almost all other cells in the tumor. OSCC cells with high expression of stemness molecules should partially behave exactly like stem cells.

口服鳞状细胞癌（OSCC）包括细胞异质性，如果它们是由茎或祖细胞失调而不是突变细胞的简单克隆膨胀而产生的，那是预期的。但是，干性分子表达水平和分布尚不清楚。为了克服这个问题，在OSCC及其特性和预后确定了干分子表达式。通过微阵列在12个肿瘤区域和OSCC的背景对病例中鉴定了两个染色质调节剂BMI-1和HMGA2。已知这两个分子都通过负调节Ink4a和ARF肿瘤抑制剂的表达来促进干细胞自我更新。在QRT-PCR和91例同一舌scc的情况下，分析了38对OSCC的表达水平。尽管有类似的靶标，但BMI-1蛋白在早期的癌变区域表达，HMGA2蛋白在进步的区域表达。同样，表达BMI1的肿瘤在总体存活方面没有显着性（P = 0.67），而表达HMGA2的肿瘤的总生存率降低（P = 0.05）。 QRT-PCR分析也与蛋白质水平相关。这些发现表明，BMI-1是将癌性与非癌区区分开的早期检测标记，而HMGA2被认为是肿瘤预后标记。在我们的OSCC分析中，这些干性分子在肿瘤中表达的原始稀有细胞不如肿瘤中的几乎所有其他细胞。具有高干性分子表达高表达的OSCC细胞应部分表现与干细胞一样。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Involvement of hepatocellular carcinoma biomarker, cyclase-associated protein 2 in zebrafish body development and cancer progression

DOI：
10.1016/j.yexcr.2012.09.013
发表时间：
2013-01-01
期刊：
EXPERIMENTAL CELL RESEARCH
影响因子：
3.7
作者：
Effendi, Kathryn;Yamazaki, Ken;Sakamoto, Michiie
通讯作者：
Sakamoto, Michiie

歯周基本治療における細菌叢変化と治療の指標となる細菌の検討

检查牙周基础治疗中细菌菌群的变化以及作为治疗指标的细菌

DOI：
发表时间：
2011
期刊：
日本歯科医学会誌
影响因子：
0
作者：
Shintani S;Hatori,M Toyoshima T;Kurihara Y;Kurokawa Y;Nakahira H;Ito T;Shirota T;金子明寛,織田洋武,伊藤公一,小林寅吉,山崎浩史,松野智宣,佐藤田鶴子,佐藤聡
通讯作者：
金子明寛,織田洋武,伊藤公一,小林寅吉,山崎浩史,松野智宣,佐藤田鶴子,佐藤聡

化学療法が奏功した耳下腺上皮筋上皮癌肺転移症例

化疗成功治愈腮腺肌上皮癌肺转移一例。