An attempt to deal with saturated fatty acid-induced insulin resistance by the stiffness of the extracellular matrix

尝试通过细胞外基质的硬度来应对饱和脂肪酸诱导的胰岛素抵抗

• 批准号: 22616005
• 负责人: LI Qinkai
• 金额: $ 2.91万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22616005/
• 关键词: 細胞外基質; 硬度; 脂肪細胞; 慢性炎症; 細胞外基質問; 弾性

We previously reported that 3T3-L1 adipocytes exhibit palmitate-induced proinflammatory response most prominently, when they are seeded on 2 -D matrices, which have a stiffness that correspond to that of obese adipose tissues. We then analyzed how mechanical properties of the matrices affect adipocyte functions, when they are seeded in a 3-D environment. Here we report that not only the stiffness of the matrices but also compression of the matrices regulate adipocyte functions.

我们以前报道，3 T3-L1脂肪细胞表现出棕榈酸诱导的促炎反应最突出，当它们被接种在2 -D矩阵，其具有对应于肥胖脂肪组织的刚度。然后，我们分析了基质的机械性能如何影响脂肪细胞的功能，当它们被接种在3-D环境中。在这里，我们报告说，不仅刚度的矩阵，但也压缩的矩阵调节脂肪细胞的功能。

项目成果

HB-EGF mediates 5-HT-induced insulin resistance through activation of EGF receptor-ERK1/2-mTOR pathway

HB-EGF通过激活EGF受体-ERK1/2-mTOR通路介导5-HT诱导的胰岛素抵抗

• 发表时间: 2011
• 作者: Hara Y;et al;船木真理;Qinkai Li
• 通讯作者: Qinkai Li

An involvement of Rho-ROCK pathway for sensing matrix rigidity in obesity

Rho-ROCK 通路参与感知肥胖基质刚性

• 发表时间: 2011
• 作者: Hara Y;et al;船木真理
• 通讯作者: 船木真理

The density of extracellular matrix proteins regulates inflammation and insulin signaling in adipocytes

• DOI: 10.1016/j.febslet.2010.08.033
• 发表时间: 2010-10-08
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Li, Qinkai;Hata, Akiko;Funaki, Makoto
• 通讯作者: Funaki, Makoto

Activation of Akt through 5-HT2A receptor ameliorates serotonin-induced degradation of insulin receptor substrate-1 in adipocytes

• DOI: 10.1016/j.mce.2012.08.022
• 发表时间: 2013-01-05
• 期刊: MOLECULAR AND CELLULAR ENDOCRINOLOGY
• 影响因子: 4.1
• 作者: Li, Qinkai;Hosaka, Toshio;Funaki, Makoto
• 通讯作者: Funaki, Makoto

Heparin-Binding EGF-Like Growth Factor (HB-EGF) Mediates 5-HT-Induced Insulin Resistance Through Activation of EGF Receptor-ERK1/2-mTOR Pathway

• DOI: 10.1210/en.2011-1418
• 发表时间: 2012-01-01
• 期刊: ENDOCRINOLOGY
• 影响因子: 4.8
• 作者: Li, Qinkai;Hosaka, Toshio;Funaki, Makoto
• 通讯作者: Funaki, Makoto