Development of a gene amplification technique with localized heating of magnetic nanoparticles via application of alternating magnetic field

本研究的目的是开发一种通过对磁性纳米颗粒进行局部加热来进行基因扩增的技术。我们利用磁性纳米粒子的局域加热现象来控制核酸的解离。此外，胺修饰的磁性颗粒已被证明通过破坏微生物膜来灭活微生物。因此，我们建议开发一种纳米级的高通量核酸扩增技术，进一步应用于微器件。

The aim of this research is development of a technique for gene amplification by localized heating of magnetic nanoparticles. We have applied the localized heating phenomena of magnetic nanoparticles to control the nucleicacid dissociation. In addition, the amine-modified magnetic particles have been demonstrated to inactive the microbe via the damage of the microbial membrane. Therefore, we propose to develop a nano-scale high throughput nucleic acid amplification technique further application in micro devices.