Enzyme-immobilized nanoporous metals for functionalization

用于功能化的酶固定纳米多孔金属

• 批准号: 22656155
• 负责人: MABUCHI Mamoru
• 金额: $ 2.27万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22656155/
• 关键词: ナノ材料; 酵素反応; 構造・機能材料; ナノバイオ; 表面・界面物性

Enzymes were immobilized on nanoporous metals for stabilization. Laccase immobilized on nanoporous Au exhibited higher thermal stability than free laccase. Decoration of nanoporous Au with self-assembled monolayer(SAM) also enhanced the thermal stability of the immobilized laccase. SAM decoration also facilitated electron transfer between electrolyte and electrode through enzymes. A prototype biofuel cell successfully operated using enzyme-immobilized SAM-modified nanoporous Au electrodes.

将酶固定在纳米多孔金属上用于稳定。漆酶固定在纳米孔Au上比游离漆酶具有更高的热稳定性。修饰纳米孔Au的自组装膜也提高了固定化漆酶的热稳定性。SAM修饰还通过酶促进电解质与电极之间的电子转移。一个原型的生物燃料电池成功地使用酶固定SAM修饰的纳米多孔Au电极。

项目成果

Nanoporous surface fabricated on metal sheets by alloying/dealloying technique

• DOI: 10.1016/j.matlet.2010.07.046
• 发表时间: 2010-11
• 期刊: Materials Letters
• 影响因子: 3
• 作者: M. Hakamada;Y. Chino;M. Mabuchi

Enzyme electrodes stabilized by monolayer-modified nanoporous Au for biofuel cells

• DOI: 10.1007/s13404-011-0038-1
• 发表时间: 2012-01-01
• 期刊: GOLD BULLETIN
• 影响因子: 2.2
• 作者: Hakamada, Masataka;Takahashi, Masaki;Mabuchi, Mamoru
• 通讯作者: Mabuchi, Mamoru

Enhanced thermal stability of laccase immobilized on monolayer-modified nanoporous Au

• DOI: 10.1016/j.matlet.2011.08.017
• 发表时间: 2012-01-01
• 期刊: MATERIALS LETTERS
• 影响因子: 3
• 作者: Hakamada, Masataka;Takahashi, Masaki;Mabuchi, Mamoru
• 通讯作者: Mabuchi, Mamoru

Electrochemical stability of self-assembled monolayers on nanoporous Au

• DOI: 10.1039/c0cp02553d
• 发表时间: 2011-01-01
• 期刊: PHYSICAL CHEMISTRY CHEMICAL PHYSICS
• 影响因子: 3.3
• 作者: Hakamada, Masataka;Takahashi, Masaki;Mabuchi, Mamoru
• 通讯作者: Mabuchi, Mamoru

Formation of Nanoporous Structure on Pt Plate Surface by Alloying/Dealloying Technique

合金/脱合金技术在铂板表面形成纳米孔结构

• DOI: 10.2320/jinstmet.75.42
• 发表时间: 2011
• 期刊: Journal of the Japan Institute of Metals and Materials
• 影响因子: 0.5
• 作者: 袴田昌高;千野靖正;馬渕守
• 通讯作者: 馬渕守