Analysis on cell filament formation and phenotypic plasticity of Escherichia coli using micro fluid system : rapid adaptation

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Using the on-chip one cell micro fluid system, we analyzed three potential factors affecting the cell filament formation of Escherichia coli ;(1) the enemy avoidance hypothesis,(2) the cell performance enhanced at a low density, and(3) the environmental stress. Either one factor or their combinations has/have been analyzed. First of all, we developed the device, so that too many growing cells can be automatically removed from the incubating area(Patent 2011-156767). Applying the device to the Tetrahymena-E. coli co-incubation system, we observed that the cell filament of E. coli forms when we used for two standard mediums for Tetrahymena(1/10PYG and CDM), even though we did not add Tetrahymena's pheromones at all.