Coevolutionary process between legume plants and parasaites/symbicants based on phylogenetic relationship.

本研究在国外研究的基础上，结合种子-捕食者(棕榈科昆虫)和节肢动物的分子系统发育分析，探讨了“资源-消费者相互作用”中网络的进化过程。(1)豆科和豆科相互作用(A)墨西哥野生豆科和豆科昆虫我们在墨西哥的Veracruz、San-Lui Ptosi和Quertaro州调查和采集了豆科的野生果实，并用分子系统学方法记录了新出现的豆科昆虫种类。虽然宏观上已经证实了相思科、番泻科和旋毛虫之间的系统发育关系，但微观上显示了复杂的系统发育模式，并伴随着入侵宿主的转移。(B)在亚洲培育豆科和棕榈科昆虫我们调查了台湾、泰国、缅甸和印度的豆科和棕榈科昆虫之间的相互作用。系统发育分析表明，资源-消费者互动模式分为Vigna和Phaseolus两个属。以分子生物学和系统发育学为基础，分析了豆科植物与豆科植物Rhyzobium之间的寄主特异性。在编码结瘤和决定寄主特异性的nod基因家族中，我们检测到了nodd基因。NodD是一种转录因子，可被多种次生代谢物Flabonid Complex激活。因为它不能被其他软糖激活，所以它决定了法巴科和Rhyzobium的寄主专一性。

The present research investigates evolutionary processes of networks in "resource-consumer interaction", based on oversea research and molecular-phylogenetic analysis with seed-predator (bruchid insects) and Rhyzobium. Two hypothetical processes were considered : phylogenetic matching in cospeciation and host shift in invasion.(1)Fabaceae and bruchidae interaction(A)Wild Fabacae and bruchid insects in MexicoWe surveyed and collected wild fruits of Fabacae in Veracruz, San-Lui Ptosi, and Quertaro states in Mexico, and recorded emergent bruchid insect species with molecular phylogenetics. Although macro-scopic phylogenetic relationships have been confirmed between Acacia-Mimosestes, Senna-Senius, and Convolvlucae-Megacerus, micro-scopic showed complicated phylogenetic patterns with invasive host shift.(B)Cultivate Fabacae and bruchid insects in AsiaWe surveyed interactions between Fabacae and bruchid insects in Taiwan, Thailand, Myanmar, and India. The phylogenetic analysis shows that resource-consumer interactive patterns are divided into two genera Vigna and Phaseolus. Boring behavior and competitive types of hatched larvae in bruchid insects were investigated in laboratory experiments.(2)Fabacae and Rhyzobium interactionHost specificity was analyzed between Fabacae plants and Rhyzobium, based on molecular biology and phylogenetics. Among nod gene family that codes nodulation and determines host-specificity, we detected nodD gene. nodD is a transcription factor that is activated with various secondary metabolistic substances, flabonoid complex. Because it cannot be activated with other flabonoids, it determines host-specificity in Fabacae and Rhyzobium.