Development of fish iPS cells utilizing biological properties of fish

点击翻译按钮获取中文摘要

Establishment of fish iPS cells is expected to enable the development of new techniques for maintaining selected aquaculture fish strains as frozen cells. This study aims as development of fundamental techniques essential for establishing fish iPS cells. We established medaka and zebrafish transgenic lines that emit GFP fluorescence under the control by oct4 promoter. We also developed cell culture system in which mulitpotency of cells can be monitored by GFP fluorescence. In addition, two possibilities on iPS cell production were suggested. First, transfection of Minicircle DNA may transform embryonic cells to iPS cells. Second, mulitpotent cells that appear in regenerating fin may be efficiently transformed to iPS cells.