Human tooth hard tissue regeneration by mouse tooth germ

啮齿类动物具有持续生长的门牙，其牙胚具有较高的分化和再生能力，可作为牙釉质再生的来源。本研究首先通过转录组分析评估幼龄和老年小鼠牙胚的基因组表达，然后通过免疫组织学和RT-PCR检测Sox2和Oct4的表达。将来自GFP小鼠门牙尖的细胞移植到肾上皮下组织，观察到具有牙齿结构的硬组织形成。目前正在研究有效收集细胞源的方法。

Rodents have continuously growing incisors, which tooth germ might be used as a source for enamel regeneration because of its high differentiation and regeneration abilities. In this study, transcriptome analysis was first performed to evaluate the young and old mice tooth germ’s genomic expression, then Sox2 and Oct4 expressions were evaluated immunohistologically and by RT-PCR. The cells derived from the apex of the incisors of GFP mice were transplanted into the subepithelial tissue ofkidney, and hard tissue formation with tooth structure was observed. The method for efficiently collecting the cell source is now under investigation.