Stem cell marker, nestin-targeting therapy in pancreatic cancer

干细胞标记物、胰腺癌的巢蛋白靶向治疗

• 批准号: 22689038
• 负责人: MATSUDA Yoko
• 金额: $ 6.57万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22689038/
• 关键词: nestin; 膵癌; 分子標的治療薬; 浸潤; 転移; 癌幹細胞; Nestin; 分子標的治療

In the present study, we have studied the effects and mechanisms of nestin-targeting therapy in pancreatic cancers. Inhibition of nestin in pancreatic cancer cell lines suppressed cell migration, invasion, sphere-forming ability and liver metastasis. The inhibitory effects of nestin were involved in the expression levels of F-actin, E-cadherin and transcription factor 4. We found that nestin expression was regulated by the methylation of the nestin gene and by the activation of cell signaling pathway proteins such as PKR and AKT. Tail vein injections of siRNA-targeting nestin into immunodeficient mice in which human pancreatic cancer had been transplanted suppressed both primary and metastatic tumor formation. Nestin is considered a promising target for pancreatic cancers, and further studies are needed to develop nestin-targeting therapy for pancreatic cancer.

在本研究中，我们研究了巢蛋白靶向治疗胰腺癌的效果和机制。胰腺癌细胞系中巢蛋白的抑制抑制细胞迁移、侵袭、球形成能力和肝转移。巢蛋白的抑制作用与F-actin、E-cadherin和转录因子4的表达水平有关。我们发现巢蛋白的表达受巢蛋白基因的甲基化和细胞信号通路蛋白如PKR和AKT的激活的调节。尾静脉注射siRNA靶向巢蛋白到免疫缺陷小鼠，其中人胰腺癌已被移植抑制原发性和转移性肿瘤的形成。巢蛋白被认为是胰腺癌治疗的一个很有前途的靶点，需要进一步研究以巢蛋白为靶点的胰腺癌治疗方法。

项目成果

Stem cell marker Nestin regulated the migration and invasion of pancreatic cancer cells.

干细胞标志物巢蛋白调节胰腺癌细胞的迁移和侵袭。

• 发表时间: 2010
• 作者: Matsuda Y;Ishiwata T;Kawamoto M;Yamomoto T;Kawahara K;Teduka T;Peng W-X;Yamahatsu K;Korc M;Naito Z.
• 通讯作者: Naito Z.

膵癌転移巣における癌幹細胞および上皮間葉転換マーカーの発現

胰腺癌转移中肿瘤干细胞和上皮间质转化标志物的表达

• 发表时间: 2012
• 作者: Kure S;Matsuda Y;Hagio M;Ueda J;Naito Z;Ishiwata T.;妹尾昌治;松田陽子
• 通讯作者: 松田陽子

Evolution of the molecular biology of brain tumors and the therapeutic inplications. Nestin: Neural stem/progenitor marker in brain tumors.

脑肿瘤分子生物学的演变及其治疗意义。

• 发表时间: 2013
• 作者: Matsuda Y;Ishiwata T;Kawamoto M;Yamomoto T;Kawahara K;Teduka T;Peng W-X;Yamahatsu K;Korc M;Naito Z.;杉本真樹;Matsuda Y
• 通讯作者: Matsuda Y

Ultra-high-resolution Images of Nestin and Vimentin in Pancreatic Carcinoma Cells Using 2 Novel Microscopy Systems

使用 2 种新型显微镜系统获得胰腺癌细胞中巢蛋白和波形蛋白的超高分辨率图像

• DOI: 10.1272/jnms.79.392
• 发表时间: 2012
• 期刊: Journal of Nippon Medical School
• 影响因子: 1
• 作者: Yoshimura H;Matsuda Y;Naito Z;Murase M;Kawamoto Y;Ishiwata T
• 通讯作者: Ishiwata T

Nestin regulates stem cell functions of pancreatic cancer cells

Nestin调节胰腺癌细胞的干细胞功能

• 发表时间: 2012
• 作者: Matsuda Y;et al.
• 通讯作者: et al.