Application of Fluorescent Metallo-Glycolipid for Targeting CancerCells

设计并合成了一种新型的金属糖脂，用于制备具有生物膜结合潜力的荧光糖探针。成功地将金属糖脂与碳水化合物一起置于仿生脂质体膜上形成荧光脂质体。脂质体用于评价糖对凝集素的识别能力。通过荧光偏振分析的亲和性评价，证明了凝集素与脂质体表面糖之间的特异性结合事件。荧光显微镜下观察到HeLa细胞对金属糖簇的摄取。这些结果表明金属糖脂可应用于癌细胞的靶向剂。

A novel metallo-glycolipid was designed and synthesized for giving a fluorescent carbohydrate probe that was potentially incorporated into a biological membrane. The metallo-glycolipid was succeeded in giving a fluorescent liposome with carbohydrates placed on a biomimetic liposomal membrane. The liposome was used for evaluateing recognition ability of carbohydrate to lectin. Specific binding events between lectin and carbohydrates on the liposomal surface was proved by the affinity evaluation with a fluorescent polarization analysis. Moreover, uptake of the metallo-glycocluster by HeLa cell was observed by fluorescent microscopy. These results suggest application of the metallo-glycolipid to a targeting agent for a cancer cell.