Comprehensive analyses of the nucleotide excision repair (NER) molecular mechanisms

核苷酸切除修复（NER）是一种多功能的DNA修复系统。进行sirna筛选和下一代DNA测序以确定新的NER因素。我们发现uvsa基因（以前称为KIAA1530）参与转录偶联核苷酸切除修复（TC-NER）。uvsa蛋白与TC-NER机制相互作用，促进DNA损伤位点的RNA聚合酶IIo泛素化。

Nucleotide excision repair (NER) is a versatile DNA repair system. SiRNA-screening and next generation DNA sequencing were performed to identify novel NER factors. We found that the UVSSA gene (formerly known as KIAA1530) is involved in the transcription-coupled nucleotide-excision repair (TC-NER). The UVSSA protein interacts with TC-NER machinery and facilitates ubiquitination of RNA polymerase IIo stalled at DNA damage sites.