Functional analyses of DNA polymerases in nucleotide excision repair

DNA聚合酶在核苷酸切除修复中的功能分析

• 批准号: 20810021
• 负责人: OGI Tomoo
• 金额: $ 2.11万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20810021/
• 关键词: DNA修復; ヌクレオチド除去修復

Nucleotide excision repair (NER) removes the UV photolesions from DNA. Compromised NER activity causes xeroderma pigmentosum, a skin cancer predisposition genetic disorder. The early step of NER is well established, whereas the later step, which involves repair synthesis and nick ligasion, is not well understood. Here we demonstrate that three DNA polymerases coordinately role in the NER repair synthesis.

核苷酸切除修复（NER）可以去除DNA上的紫外线光损伤。受损的NER活动导致着色性干皮病，一种皮肤癌易感性遗传疾病。NER的早期步骤已经确定，而涉及修复合成和缺口连接的后期步骤尚未得到很好的理解。在这里，我们证明了三种DNA聚合酶在NER修复合成中协同作用。

项目成果

A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU).

• DOI: 10.1093/nar/gkp023
• 发表时间: 2009-03
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Limsirichaikul S;Niimi A;Fawcett H;Lehmann A;Yamashita S;Ogi T
• 通讯作者: Ogi T

日本国特許

日本专利

• 发表时间: 2006

A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethyny1 deoxyuridine (EdU)

一种通过掺入乙炔脱氧尿苷 (EdU) 测量原代人成纤维细胞修复合成的快速非放射性技术

• 发表时间: 2009
• 期刊: Nucleic Acids Research 37
• 作者: Limsirichaikul;et al.
• 通讯作者: et al.

損傷DNA修復物質のスクリーニング方法

损伤DNA修复物质的筛选方法

• 发表时间: 2009

Three DNA Polymerases, Recruited by Different Mechanisms, Carry Out NER Repair Synthesis in Human Cells

• DOI: 10.1016/j.molcel.2010.02.009
• 发表时间: 2010-03-12
• 期刊: MOLECULAR CELL
• 影响因子: 16
• 作者: Ogi, Tomoo;Limsirichaikul, Siripan;Lehmann, Alan R.
• 通讯作者: Lehmann, Alan R.