Mechanism of histone dimethylation regulating DNA damage response

组蛋白二甲基化调节DNA损伤反应的机制

• 批准号: 23310038
• 负责人: SUZUKI Keiji
• 金额: $ 12.15万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23310038/
• 关键词: 放射線; DNA; クロマチン; ヒストン; メチル化; DNA損傷; 情報伝達; G9a蛋白質

To secure genome integrity of the cells DNA damage signals are amplified and persists, if DNA double strand breaks are remained. This study demonstrated that , G9a, a histone H3 dimethylase, is involved in persisted DNA damage signal amplification. G9a was found to be relocated to the chromatin regions harboring DNA damage, and it executed dimethylation of histone H3 at Lysine 9. Dimethylated histone H3 at Lysine 9 was recognized by 53BP1 through its so-called Tudor domain, thereby amplified 53BP1 foci formation was maintained. The study indicated that dimethylation of histone H3 at Lysine 9 by recruted G9a to DNA damage sites is essential for the persistence of amplified DNA damage signals.

为了确保细胞基因组的完整性，DNA损伤信号被放大并持续存在，如果DNA双链断裂仍然存在。本研究表明，组蛋白H3二甲基化酶G9a参与持续DNA损伤信号扩增。G9a被发现重新定位到含有DNA损伤的染色质区域，并在赖氨酸9处执行组蛋白H3的二甲基化。Lysine 9的二甲基化组蛋白H3通过Tudor结构域被53BP1识别，从而维持了53BP1扩增灶的形成。该研究表明，G9a在DNA损伤位点对赖氨酸9的组蛋白H3的二甲基化对于DNA损伤信号扩增的持久性至关重要。

项目成果

Clustered DNA damage and ATM-dependent DNA damage response

聚集性 DNA 损伤和 ATM 依赖性 DNA 损伤反应

• 发表时间: 2012
• 作者: Satoshi Nakano;Ayu Washizu;柴崎茂光・愛甲哲也・池田優佑・枚田邦宏;深見聡;植田和弘・山口臨太郎;鈴木啓司
• 通讯作者: 鈴木啓司

原研医療

哈拉肯医疗

Increased susceptibility to delayed genetic effects of low dose X-irradiation in DNA repair deficient cells

DNA 修复缺陷细胞对低剂量 X 射线照射延迟遗传效应的敏感性增加

• DOI: 10.3109/09553002.2013.752596
• 发表时间: 2013
• 期刊: Int J Radiat Biol.
• 作者: Kashino G;Suzuki K;Kodama S;Watanabe M;Prise KM
• 通讯作者: Prise KM

Singnificance of p53-binding protein 1 (53BP1) expression in thyroid papillary microcarcinoma : association with BRAFV600E mutation status

p53 结合蛋白 1 (53BP1) 在甲状腺微小乳头状癌中表达的意义：与 BRAFV600E 突变状态的关联

• 发表时间: 2013
• 期刊: Histopathology
• 影响因子: 6.4
• 作者: Mussazhanova Z;Matsuda K;Naruke Y;Mitsutake N;Stanojevic B;Rogounovitch T;Saenko V;Suzuki K;Nishihara E;Hirokawa M;Ito M;Nakashima M
• 通讯作者: Nakashima M

Mode of ATM-dependent suppression of chromosome translocation.

ATM 依赖性染色体易位抑制模式。

• DOI: 10.1016/j.bbrc.2011.11.006
• 发表时间: 2011
• 期刊: Biochem Biophys Res Commun
• 作者: Yamauchi M;Suzuki K;Oka Y;Suzuki M;Kondo H;Yamashita S
• 通讯作者: Yamashita S