Enhancement of local electric field in the atto-liter space and its application for ultra sensitive screening tips

在这项研究中，它的目的是制造一个尺寸在亚波长以下的可控原子空间，并将等离子体共振场限制在空间中，以实现超高灵敏的生物传感。然后，我们得到了一种独特的以聚苯乙烯球形粒子为模板的结构制备方法，以及利用纳米印迹技术进行结构复制的合适条件，并证明了局部表面等离子体芯片即使在含有一定污染物的样品中也表现出了超高的灵敏度。此外，我们还开发了一个由掌上型光学测量仪器和远程操作终端组成的原型测试系统，这是本项目原计划中没有的，并成功地澄清了实际应用的实际图像。该研究成果将实现生物筛选检测和护理点检测，具有无标签、简单、超高灵敏度的特点。

In this research, it have aimed to fabricate a controlled atto-liter space whose dimension below a sub-wavelength and to confine a plasmon resonance field into the space in order to achieve ultrahigh-sensitive biosensing. Then, we obtained a unique structure fabrication method templating polystylene spherical particles and an appropriate condition for structure replication via nanoimprinting process, and also proved that the local surface plasmon chip exhibits ultra-high sensitivity even for the sample containing a certain contaminant. Moreover, we have developed a prototype testing system consists of palmtop optical measurement apparatus and remote operation terminal that was not included in the original plan of this project, and succeeded to clarify a practical image of the real application. The outcome of the research will lead to the realization of bio-screening testing and point-of-care testing with a feature of label-free, simple, and ultra-high sensitivity.