Development of a new protein crystallization technique by the femtosecond laser irradiation at gel-solution or air-solution interfaces

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We developed a new protein crystallization technique by the femtosecond laser irradiation at gel-solution or air-solution interfaces.The laser irradiation near the interfaces under optimized conditions could induce the nucleation at very low supersaturation, where no nucleation could be induced even by the femtosecond laser irradiation of bulk solutions. We applied solution-stirring technique to the crystals nucleated at gel-solution interfaces, and succeeded to improve crystal quality.