Research on signal transduction via transcriptional mediators

转录介质信号转导研究

• 批准号: 23370079
• 负责人: ISHII Shunsuke
• 金额: $ 12.81万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23370079/
• 关键词: 転写メディエーター; シグナル伝達; 転写アダプター; TRIM27; Shn-2; 転写制御; メディエーター; 転写; T細胞; 分化; アポトーシス

We found that corepressor Ski binds to TRIM27, and that TRIM27 contributes to deubiquitination via forming a complex with USP7. It was found that TRIM27 enters into the nucleus in response to poly(I-C) stimulation, suggesting that Ski ubiquitination may be regulated by TRIM27 in response to specific signaling.In knockout mice of transcriptional adaptor Shn-2, T cell differentiation in thymus is blocked. It was found that Shn-2 is modified by O-GlcNAc at several sites which modulates nuclear entry of Shn-2. It was demonstrated that Shn-2 is localized on the T-cell receptor beta gene locus, and modulated allelic exclusion.

我们发现辅阻遏物Ski与TRIM 27结合，并且TRIM 27通过与USP 7形成复合物而有助于去泛素化。研究发现TRIM 27在poly（I-C）刺激下进入细胞核，提示TRIM 27可能通过特异性信号调节Ski泛素化。发现Shn-2在几个位点被O-GlcNAc修饰，其调节Shn-2的核进入。结果表明，Shn-2定位于T细胞受体β基因座，并调节等位基因排斥。

项目成果

Ubiquitination-deubiquitination by the TRIM27-USP7 complex regulates TNF-α-induced apoptosis

TRIM27-USP7 复合物的泛素化-去泛素化调节 TNF-α 诱导的细胞凋亡

• 发表时间: 2013
• 作者: Zaman MM;Ishii S 他
• 通讯作者: Ishii S 他

Inheritance of stress-induced epigenome change : research using fly and mouse

应激引起的表观基因组变化的遗传：使用果蝇和小鼠的研究

• 发表时间: 2011
• 作者: Staton TL;Lazarevic V;Jones DC;Lanser AJ;Takagi T;Ishii S;Glimcher LH;石井俊輔
• 通讯作者: 石井俊輔

Ubiquitination-deubiquitination by the TRIM27-USP7 complex regulates TNF- α-induced apoptosis

TRIM27-USP7 复合物的泛素化-去泛素化调节 TNF-α 诱导的细胞凋亡

• 发表时间: 2013
• 作者: Zaman MM;Nomura T;Takagi T;Okamura T;Jin W;Shinagawa T;Tanaka Y;Ishii S
• 通讯作者: Ishii S

Inheritance of Stress-Induced, ATF-2-Dependent Epigenetic Change

• DOI: 10.1016/j.cell.2011.05.029
• 发表时间: 2011-06-24
• 期刊: CELL
• 影响因子: 64.5
• 作者: Seong, Ki-Hyeon;Li, Dong;Ishii, Shunsuke
• 通讯作者: Ishii, Shunsuke

ATF-7 controls epigenetic memory of pathogen infection in macrophages.

ATF-7 控制巨噬细胞中病原体感染的表观遗传记忆。

• 发表时间: 2013
• 作者: Yoshida K;Ishii S 他
• 通讯作者: Ishii S 他