Development of precision livestock oriented high-speed serum vitamin A prediction method to produce high-quality beef with high marbling standard

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To produce high-quality beef with high marbling standard, Japanese Black cattle farmers usually manipulate the serum vitamin A in cattle at a minimum desired low level. In this study, we investigated experimentally on the estimation of the serum vitamin A by processing the pupil image. Pupil images of Japanese Black cattle were taken with a 2CCD camera. A fundus camera was also used. Blood assay was done to measure the serum vitamin A level. The result showed that the blue component ratio of the pupil image of cattle with lower vitamin A level tended to be small. Multivariate classification method using five variables (such as the red component ratio and intensity) was used. The result showed that mild and severe vitamin A deficiency groups could be classified with high classification rate. For the practical use, an automatic imaging equipment that can capture the pupil image frequently was made and tested.