The mechanism of activation of Langerhans cells mediated by keratinocytes

角质形成细胞介导的朗格汉斯细胞活化机制

• 批准号: 23380184
• 负责人: MAEDA SADATOSHI
• 金额: $ 11.65万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380184/
• 关键词: アレルギー・ぜんそく; 獣医学; トランスレーショナルリサーチ; アトピー性皮膚炎; ケラチノサイト

The results indicated that the stimulation with house dust mite antigen (HDM) induced the production of GM-CSF in canine keratinocytes, which was suppressed by a cysteine protease inhibitor. Thus, it was demonstrated that cysteine protease activity was essential for GM-CSF production in canine kerationocytes activated by HDM.Papain that was a naturally derived cysteine protease induced nuclear translocation of NFAT. GM-CSF production was partially inhibited by blockade of NFAT with ciclosporin. These results suggest that GM-CSF production mediated by the cysteine protease is regulated not only by NFAT but also by unknown signaling pathways in canine keratinocytes.

结果表明，室内粉尘螨抗原（HDM）的刺激诱导了犬角质形成细胞中GM-CSF的产生，该犬被半胱氨酸蛋白酶抑制剂抑制。 因此，已经证明半胱氨酸蛋白酶活性对于由HDM.Papain激活的犬keration核细胞中的GM-CSF产生至关重要，这是一种天然衍生的半胱氨酸蛋白酶诱导NFAT的核转运。 GM-CSF的产生通过Ciclosporin对NFAT的阻滞部分抑制。 这些结果表明，由半胱氨酸蛋白酶介导的GM-CSF产生不仅受NFAT调节，而且还通过犬角质形成细胞中未知的信号通路来调节。

项目成果

Production of GM-CSF Mediated by Cysteine Protease of Der f 1 in Canine Keratinocytes

犬角质形成细胞中 Der f 1 半胱氨酸蛋白酶介导的 GM-CSF 产生

• 发表时间: 2012
• 期刊: Journal of Veterinary Medical Science
• 影响因子: 1.2
• 作者: Tsukamura;H.;Tomikawa;J.;Uenoyama;J.;Maeda;K.-I.;Tsuyoshi KIMURA
• 通讯作者: Tsuyoshi KIMURA

Production of GM-CSF Mediated by Cysteine Protease of Der f in Canine Keratinocytes

• DOI: 10.1292/jvms.11-0522
• 发表时间: 2012-08-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Kimura, Tsuyoshi;Sekido, Machiko;Maeda, Sadatoshi
• 通讯作者: Maeda, Sadatoshi

犬アトピー性皮膚炎のサイトカイン・ケモカイン

犬特应性皮炎的细胞因子和趋化因子

• 作者: Chimura;N.;Kondo;N.;Shibata;S.;Kimura;T.;Mori;T.;Hoshino;Y.;Murayama;N.;Nagata;M.;Ide;K.;Nishifuji;K.;Kamishina;H.;Maeda;S;前田貞俊
• 通讯作者: 前田貞俊

Protease-activated receptor-2 induces proinflammatory cytokine and chemokine gene expression in canine keratinocytes

• DOI: 10.1016/j.vetimm.2013.01.018
• 发表时间: 2013-05-15
• 期刊: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
• 影响因子: 1.8
• 作者: Maeda, Shingo;Maeda, Sadatoshi;Tsujimoto, Hajime
• 通讯作者: Tsujimoto, Hajime

Involvement of nuclear factor of activated T cells in granulocyte-macrophage colony-stimulating factor production in canine keratinocytes stimulated with a cysteine protease

半胱氨酸蛋白酶刺激的犬角质形成细胞中活化 T 细胞的核因子参与粒细胞-巨噬细胞集落刺激因子的产生

• DOI: 10.1111/vde.12017
• 发表时间: 2013
• 期刊: Vet Dermatol
• 影响因子: 1.4
• 作者: Kimura;T.;Sekido;M.;Iio;A.;Chimura;N.;Shibata;S.;Kamishina H.;Maeda;S
• 通讯作者: S