Development of targeted medical care (simultaneous diagnostic imaging and therapy) for pathogenic angiogenesis using fibrinolytic metabolites

利用纤溶代谢物开发针对致病性血管生成的靶向医疗护理（同时诊断成像和治疗）

• 批准号: 23390151
• 负责人: MATSUURA Eiji
• 金额: $ 4.24万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-11-18 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390151/
• 关键词: 血管新生; ベータ2-グリコプロテインI; 抗血管新生剤; B2GPI-ドメインI; nicked B2GPI; PETイメージング; 質量分析; DDSキャリア; beta2 glycoprotein I; B2GPI-DI; VEGF; β2GPI; nicked-β2GPI; β2GPI-DI; DOTA修飾; VEGF受容体; MALDI-TOF-MS; β2-グリコプロテインI(β2GPI); β2GPI-DV; nicked DV; 抗血管新生; マイ

By the cytostatic activity test and angiogenesis imaging analysis with human umbilical vein endothelial cells, we confirmed that DI of beta2-glycoprotein I (B2GPI) revealed dose- and time-dependent cytostatic and anti-angiogenesis activity. 64Cu-labeled DI and 64Cu-labeled B2GPI were prepared and used for PET/CT imaging of tumor-bearing mice. DI showed high tumor/blood ratio. 64Cu-labeled and B2GPI-bound liposomes were prepared as an experimental model of the Theranostics (simultaneous diagnostic imaging and therapy) technology.

通过人脐静脉内皮细胞的细胞抑制活性试验和血管生成成像分析，我们证实了β2-糖蛋白I(B2GPI)的DI具有剂量和时间依赖性的细胞抑制和抗血管生成活性。制备了64铜标记的DI和64铜标记的B2GPI，并用于荷瘤小鼠的PET/CT显像。DI显示肿瘤/血液比值较高。制备了64Cu标记和B2GPI结合的脂质体，作为Theranostics(同时诊断成像和治疗)技术的实验模型。

项目成果

Recombinant domain V of β2-glycoprotein I inhibits the formation of a 7-ketocholesteryl-9-carboxynonanoate and β2-glycoprotein I complex.

• DOI: 10.1093/jb/mvq111
• 发表时间: 2011
• 期刊: Journal of biochemistry
• 影响因子: 2.7
• 作者: Yingbiao Zhang;Wenzhe Li;Y. Chi;Ren-jun Wang;Dan-Bing Wang;Fan Zhang;Zhe Liu;E. Matsuura;Qing-ping Liu

In vivo oxidation, platelet activation and simultaneous occurrence of natural immunity in atherosclerosis-prone mice.

易患动脉粥样硬化的小鼠体内氧化、血小板活化和自然免疫同时发生。

• 发表时间: 2011
• 期刊: The Israel Medical Association Journal
• 作者: Shen L;Matsunami Y;Quan N;Kobayashi K;Matsuura E;Oguma K.
• 通讯作者: Oguma K.

A non-invasive PET/CT imaging procedure for diagnosing atherosclerotic lesions : immunologic targeting to oxLDL/beta2-glycoprotein I in the WHHL rabbits

用于诊断动脉粥样硬化病变的非侵入性 PET/CT 成像程序：WHHL 兔中 oxLDL/β2-糖蛋白 I 的免疫靶向

• 发表时间: 2013
• 作者: Sasaki T;Matsunami Y;Matsuura E
• 通讯作者: Matsuura E

A Possible Mechanism of Autoimmune-Mediated infertility in Women with Endometriosis

• DOI: 10.1111/j.1600-0897.2010.00956.x
• 发表时间: 2011-08-01
• 期刊: AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
• 影响因子: 3.6
• 作者: Inagaki, Junko;Hao, Lin;Matsuura, Eiji
• 通讯作者: Matsuura, Eiji

In vivo molecular imaging using humanized anti-beta2-glycoprotein I autoantibodies to detect arterial lesions in the atherosclerosis prone apoE-/- mice

使用人源化抗 β2-糖蛋白 I 自身抗体进行体内分子成像检测易患动脉粥样硬化的 apoE-/- 小鼠的动脉病变

• 发表时间: 2014
• 作者: Matsunami Y;Matsuura E;et al(他5名)
• 通讯作者: et al(他5名)