Development of targeted therapy of cancer cells with diagnostic imaging through the application of immunoliposomes

通过免疫脂质体的应用开发诊断成像的癌细胞靶向治疗

• 批准号: 23659299
• 负责人: MATSUURA Eiji
• 金额: $ 2.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659299/
• 关键词: 分子イメージング; Immuno-Theranostics; がん; メソセリン; リポソーム

The present study was aimed to develop a monoclonal antibody-based probe for PET and drug delivery targeting mesothelin (MSLN) expressing cancer, through the application of immunoliposomes. A humanized monoclonalantibody was developed from a mouse monoclonal antibody against MSLN. We also started to develop its single-chain variable fragment (scFv). In vivo mouse PET imaging with 64Cu-lableld Fab fragment of anti-MSLN antibody showed faster tumor targeting than the case with 64Cu-labeled anti-MSLN IgG. Liposomes conjugated with humanized anti-MSLN antibody and labeled with Cy5.5 were taken into MSLN-expressing cultured cancer cells in an antibody-dependent manner.

本研究旨在通过免疫脂质体的应用，开发用于PET和靶向表达间皮素（MSLN）的癌症的药物递送的基于单克隆抗体的探针。从抗MSLN的鼠源单克隆抗体出发，研制了人源化的单克隆抗体。我们也开始开发其单链可变区片段（scFv）。用64Cu标记的抗MSLN抗体Fab片段的体内小鼠PET成像显示出比用64Cu标记的抗MSLN IgG的情况更快的肿瘤靶向。将与人源化抗MSLN抗体缀合并用Cy5.5标记的脂质体以抗体依赖性方式引入表达MSLN的培养的癌细胞中。

项目成果

Persimmon (Diospyros Kaki Thunb ‘Saijo’) peel improved dyslipidemia and its related production of atherogenic autoantigen complexes in low-density lipoprotein receptor-deficient mice

柿子（Diospyros Kaki Thunb ‘Saijo’）皮改善低密度脂蛋白受体缺陷小鼠的血脂异常及其相关的致动脉粥样硬化自身抗原复合物的产生

• DOI: 10.1136/heartjnl-2011-300867.49
• 发表时间: 2011
• 期刊: Heart
• 影响因子: 5.7
• 作者: N. Quan

On aspirin treatment but not baseline thromboxane B2 levels predict adverse outcomes in patients with acute coronary syndromes

阿司匹林治疗而非基线血栓素 B2 水平可预测急性冠脉综合征患者的不良后果

• DOI: 10.1111/j.1538-7836.2012.04845.x
• 发表时间: 2012
• 期刊: J Thromb Haemost
• 影响因子: 10.4
• 作者: Ohta H;Iwamoto S;et al.;Eiji Matsuura
• 通讯作者: Eiji Matsuura

Lopez. Autoimmunity, oxidative inflammation, and b2-glycoprotein I in early athereogenesis

洛佩兹。

• 发表时间: 2013
• 作者: Ohta H;Iwamoto S;et al;E. Matsuura,L.R. Lopez.
• 通讯作者: E. Matsuura,L.R. Lopez.

抗体を用いる標的医療:動脈硬化、がんへの新たな取り組み

使用抗体的靶向药物：治疗动脉硬化和癌症的新方法

• 发表时间: 2012
• 作者: Mizuike;R.;Sasaki;T;Baba;K.;Iwamoto;H.;Shibai;Y.;Kosaka;M.;Kubota-Koketsu;R.;Yang;C.-S.;Du;A.;Sakudo;A.;Tsujikawa;M.;Yunoki;M.;Ikuta;K;松浦栄次
• 通讯作者: 松浦栄次

ホームページ(岡山大学 産学官連携センター「おかやまメディカルイノベーションセンター(OMIC)」

主页（冈山大学产学官协作中心“冈山医疗创新中心（OMIC）”）