Treatment strategy and analysis of progression mechanism of musculoskeletal tumors using fluorescent imaging

肌肉骨骼肿瘤的荧光成像治疗策略及进展机制分析

• 批准号: 23390360
• 负责人: TSUCHIYA Hiroyuki
• 金额: $ 10.07万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390360/
• 关键词: 骨・軟部腫瘍学; 癌; 蛍光イメージング

Fluorescent labeled osteosarcoma cell line was transplanted in nude mice and harvested in lung metastasis to obtain higher metastatic cell line. The more metastatic potential, the more PAI -1 and u-PA levels increased. We previously reported that caffeine-assisted chemotherapy. We found caffeine affects tumor cells through various pathways, such as PTEN, AKT, NF-kB, and ERK activities. Modulation of cisplatinum efficacy induced by caffeine was visualized at the subcellular level by fluorescent ubiquitination-based cell cycle indicator in the nucleus. Caffeine overcame the cell-cycle arrest induced by cisplatinum, thereby increasing its efficacy. Theobromine, a caffeine derivative, was investigated as a new anti-cancer effect. It elevates intracellular cAMP levels and inhibits cell proliferation. Surgical navigation for bone and soft tissue tumor in mouse and human was also performed using ICG and near-infrared fluorescent imaging.

将荧光标记的骨肉瘤细胞株移植于裸鼠体内，并在肺转移处收获，以获得高转移细胞株。派-1和u-PA水平随转移潜能的增加而升高。我们以前报道过咖啡因辅助化疗。我们发现咖啡因通过多种途径影响肿瘤细胞，如PTEN，AKT，NF-kB和ERK活性。咖啡因诱导的顺铂疗效的调制可视化在亚细胞水平的细胞核中的荧光泛素化为基础的细胞周期指示剂。咖啡因克服了顺铂引起的细胞周期停滞，从而增加了其疗效。可可碱是一种咖啡因衍生物，被研究为一种新的抗癌作用。它提高细胞内cAMP水平并抑制细胞增殖。还使用ICG和近红外荧光成像对小鼠和人的骨和软组织肿瘤进行手术导航。

项目成果

腫瘍再建術の最前線

肿瘤重建手术的最前沿

• 发表时间: 2012
• 作者: Hideji Nishida;Yoshikazu Tanzawa;Norio Yamamoto;Katsuhiro Hayashi;Akihiko Takeuchi;Hiroaki Kimura;Shingo Shimozaki;Hiroyuki Tsuchiya;土屋弘行;Tsuchiya H;Tsuchiya H;土屋弘行;土屋弘行
• 通讯作者: 土屋弘行

患肢温存手術の動向-そして未来へ

保肢手术的趋势和未来

• 发表时间: 2012
• 作者: Muraki S;Akune T;Oka H;Nakamura K;Kawaguchi H;Yoshimura N;Yamaji H et al.,;島津洋介;平田雅之;土屋弘行
• 通讯作者: 土屋弘行

Benefit of caffeine-potentiated chemotherapy for high-grade soft tissue sarcoma

咖啡因强化化疗对高级别软组织肉瘤的益处

• 发表时间: 2011
• 作者: Takeuchi A;Tsuchiya H;et al
• 通讯作者: et al

Biological reconstruction: Kanazawa experience

生物重建：金泽经验

• 作者: 北圭介;中田研;前達雄;中村憲正;松尾知彦;金銅真世;太田啓介;吉川秀樹;Tsuchiya H
• 通讯作者: Tsuchiya H

Potentiation of the antitumor effect of calcium phosphate cement containing anticancer drug and caffeine on rat osteosarcoma

• DOI: 10.1007/s00776-011-0045-3
• 发表时间: 2011
• 期刊: Journal of Orthopaedic Science
• 影响因子: 1.7
• 作者: Y. Tanzawa;H. Tsuchiya;T. Shirai;H. Nishida;K. Hayashi;A. Takeuchi;M. Kawahara;K. Tomita