Treatment strategy and analysis of progression mechanism of musculoskeletal tumors using fluorescent imaging

将荧光标记的骨肉瘤细胞株移植到裸鼠体内，在肺转移中收获，获得高转移性细胞系。转移潜能越大，PAI-1和u-PA水平升高越明显。我们之前曾报道过咖啡因辅助化疗。我们发现咖啡因通过多种途径影响肿瘤细胞，如PTEN、AKT、NF-kB和ERK活性。通过细胞核内基于荧光泛素化的细胞周期指示剂，在亚细胞水平上可视化了咖啡因对顺铂效应的调节。咖啡因克服了顺铂引起的细胞周期停滞，从而提高了其疗效。可可碱是一种咖啡因衍生物，是一种新的抗癌活性物质。它能提高细胞内cAMP水平，抑制细胞增殖。应用ICG和近红外荧光成像技术对小鼠骨和软组织肿瘤进行手术导航。

Fluorescent labeled osteosarcoma cell line was transplanted in nude mice and harvested in lung metastasis to obtain higher metastatic cell line. The more metastatic potential, the more PAI -1 and u-PA levels increased. We previously reported that caffeine-assisted chemotherapy. We found caffeine affects tumor cells through various pathways, such as PTEN, AKT, NF-kB, and ERK activities. Modulation of cisplatinum efficacy induced by caffeine was visualized at the subcellular level by fluorescent ubiquitination-based cell cycle indicator in the nucleus. Caffeine overcame the cell-cycle arrest induced by cisplatinum, thereby increasing its efficacy. Theobromine, a caffeine derivative, was investigated as a new anti-cancer effect. It elevates intracellular cAMP levels and inhibits cell proliferation. Surgical navigation for bone and soft tissue tumor in mouse and human was also performed using ICG and near-infrared fluorescent imaging.