Treatment strategy and analysis of progression mechanism of musculoskeletal tumors using fluorescent imaging

肌肉骨骼肿瘤的荧光成像治疗策略及进展机制分析

• 批准号: 23390360
• 负责人: TSUCHIYA Hiroyuki
• 金额: $ 10.07万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390360/
• 关键词: 骨・軟部腫瘍学; 癌; 蛍光イメージング

Fluorescent labeled osteosarcoma cell line was transplanted in nude mice and harvested in lung metastasis to obtain higher metastatic cell line. The more metastatic potential, the more PAI -1 and u-PA levels increased. We previously reported that caffeine-assisted chemotherapy. We found caffeine affects tumor cells through various pathways, such as PTEN, AKT, NF-kB, and ERK activities. Modulation of cisplatinum efficacy induced by caffeine was visualized at the subcellular level by fluorescent ubiquitination-based cell cycle indicator in the nucleus. Caffeine overcame the cell-cycle arrest induced by cisplatinum, thereby increasing its efficacy. Theobromine, a caffeine derivative, was investigated as a new anti-cancer effect. It elevates intracellular cAMP levels and inhibits cell proliferation. Surgical navigation for bone and soft tissue tumor in mouse and human was also performed using ICG and near-infrared fluorescent imaging.

将标记为骨肉瘤的荧光细胞系移植在裸鼠中，并在肺转移中收获以获得较高的转移细胞系。转移潜力越多，PAI -1和U -PA水平越多。我们先前报道了咖啡因辅助化疗。我们发现咖啡因通过PTEN，AKT，NF-KB和ERK活动等各种途径影响肿瘤细胞。通过基于荧光泛素化的细胞周期指标，在亚细胞水平上可视化咖啡因诱导的顺铂疗效的调节。咖啡因克服了顺铂诱导的细胞周期停滞，从而提高了其功效。 Theobromine是一种咖啡因衍生物，被研究为一种新的抗癌作用。它提高了细胞内营地水平并抑制细胞增殖。还使用ICG和近红外荧光成像进行了小鼠和人类骨骼和软组织肿瘤的手术导航。

项目成果

蛍光蛋白を用いたがん細胞のin vivo 動態イメージング

使用荧光蛋白对癌细胞进行体内动态成像

• 发表时间: 2011
• 作者: 山本憲男;土屋弘行;他
• 通讯作者: 他

抗β1 インテグリン抗体は骨肉腫細胞の肺への接着および肺転移の発生を抑制する

抗β1整合素抗体抑制骨肉瘤细胞对肺的粘附和肺转移的发展

• 发表时间: 2011
• 作者: 木村浩明;土屋弘行;他
• 通讯作者: 他

Joint-preserving reconstruction of metaphyseal malignant bone tumors using tumor-bearing massive bone treated with liquid nitrogen.

使用液氮处理的荷瘤大块骨进行干骺端恶性骨肿瘤的保关节重建。

• 发表时间: 2012
• 作者: Cilek MZ;et al.;Tsuchiya H
• 通讯作者: Tsuchiya H

Long-term outcomes of osteosarcoma patients with a complete response to chemotherapy and who subsequently underwent intentional marginal resection

对化疗完全缓解并随后接受有意边缘切除的骨肉瘤患者的长期结果

• 发表时间: 2011
• 作者: Yamamoto N;Tsuchiya H;et al
• 通讯作者: et al

骨肉腫に対する挑戦

对抗骨肉瘤的挑战

• 发表时间: 2013
• 作者: Hasegawa M;Sudo A.;Yoshimura N;土屋弘行
• 通讯作者: 土屋弘行