Fluorescent in vivo imaging of bone and soft tissue tumor -primary, metastasis and therapy-

我们研究了体内肉瘤荧光成像。荧光碱性磷酸酶抗体可标记骨肉瘤。虽然GFP（绿色）被广泛用于荧光成像，但它存在自身荧光的问题。红光或红外线较好地检测无自身荧光的细胞。通过对小鼠肺转移模型的实时成像，观察免疫细胞在早期转移中的作用。树突状细胞免疫治疗联合肿瘤裂解液冷冻治疗是有效的。

We have investigated in vivo sarcoma fluorescent imaging. Fluorescent alkaline phosphatase antibody can label osteosarcoma. Although GFP (green) is widely used for fluorescent imaging, it has a problem of autofluorescence. Red or infrared were better observed to detect cells without autofluorescence. Real-time imaging performed in mouse lung metastasis model, the contribution of immune cells for early step of metastasis was observed. Dendritic cells for immunotherapy combined with tumor lysate of cryo-treatment were effective.