Altered dopaminergic system by environmental enrichment in ADHD model rat

ADHD模型大鼠环境富集改变多巴胺能系统

• 批准号: 23500395
• 负责人: HIDA HIDEKI
• 金额: $ 3.33万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23500395/
• 关键词: enrichment; emotion; growth; CART; dopamine; amygdala; ADHD; rat; 扁桃体中心核; うま味; GABA; 弧束核; 攻撃性; ドパミン受容体; ドパミン; 豊かな環境; 側坐核; 内側前頭葉; メタボローム解析; 多動性; モデルラット; コカイン; オープンフィールドテスト; 内側前頭野

To investigate the relation between environmental enrichment (EE) during the period of development and formation of emotion, spontaneously hypertensive rat (SHR) were allowed to EE for 5 weeks from P25, followed by assessment of emotional behavior and analysis in dopaminergic system focusing on cocaine and amphetamine regulated transcript (CART).In open-field test, total distance (parameter for hyperactivity) and locomotion speed (impulsivity) were deceased in EE-grown rats. In cylinder test, more quick adaptation to novelty was shown in EE. CART is enhanced by EE in the amygdala (Amy) by real-time PCR, and many CART-positive cells was observed in the Amy, especially in the central nucleus of amygdale (CeA). CART colocalized in GABAergic cells that were not immnoreactive to parvalbumin and calretinin. Data suggested that EE induced CART expression in CeA that colocalized in GABAergic neurons probably relating to decreased hyperactivity and anxiety-like behavior in SHR.

为探讨发育期环境丰富（EE）与情绪形成的关系，采用自发性高血压大鼠（SHR），从P25开始给予EE 5周，观察其情绪行为，并对多巴胺能系统进行可卡因和苯丙胺调节转录本（CART）分析。在EE-生长的大鼠中，总距离（活动过度的参数）和运动速度（冲动性）下降。实时荧光定量PCR结果显示，EE能增强杏仁核（Amy）CART反应，在Amy中观察到大量CART阳性细胞，尤其是杏仁中央核（CeA）。CART共定位于对小清蛋白和钙视网膜蛋白无免疫反应的GABA能细胞。EE诱导CART在CeA中的表达，其共定位于GABA能神经元，可能与SHR的多动和焦虑样行为减少有关。

项目成果

Oral intake of monosodium glutamate during the period of development effects on social behavior mediated by gut-brain communication in an ADHD model rat

发育期口服味精对多动症模型大鼠肠脑通讯介导的社会行为的影响

• 发表时间: 2014
• 作者: Hida H;Yokoyama Y;Marumoto R;Shimizu Y;Ueda Y;Misumi s;Ishida A;Jung C-G
• 通讯作者: Jung C-G

Cocaine- and amphetamine-regulated transcript in the central nucleus of amygdala is enhanced by environmental enrichment in ADHD model rat

ADHD 模型大鼠杏仁核中央核中可卡因和安非他明调节的转录物因环境富集而增强

• 发表时间: 2014
• 作者: Shimizu Y;Yokoyama Y;Misumi S;Ishida A;Jung C-G;Hida H
• 通讯作者: Hida H

発達期の経口うま味刺激における迷走神経を介した社会性亢進

发育过程中口腔鲜味刺激过程中迷走神经介导的社交过度活跃

• 发表时间: 2013
• 作者: 横山善弘;清水由布子;上田佳朋;三角吉代;石田章真;鄭 且均;横井基夫;飛田秀樹
• 通讯作者: 飛田秀樹

Behavioral effect of environmental enrichment on attention deficit hyperactivity disorder model rat : CART expression in the medial prefrontal cortex and the amygdala

丰富环境对注意缺陷多动障碍模型大鼠的行为影响：内侧前额皮质和杏仁核中的CART表达

• 发表时间: 2013
• 作者: Shimizu Y;Yokoyama Y;Misumi S;Ishida A;Jung CG;Hida H
• 通讯作者: Hida H

Reduced hyperactivity with CART expression in the prefrontal cortex in ADHD model rats

ADHD 模型大鼠前额皮质中 CART 表达减少多动症

• 发表时间: 2012
• 作者: Shimizu Y;Watanabe Y;Yokoyama Y;Cho R;Misumi S;Masuda T;Hida H
• 通讯作者: Hida H