Altered dopaminergic system by environmental enrichment in ADHD model rat

为探讨发育期环境丰富（EE）与情绪形成的关系，采用自发性高血压大鼠（SHR），从P25开始给予EE 5周，观察其情绪行为，并对多巴胺能系统进行可卡因和苯丙胺调节转录本（CART）分析。在EE-生长的大鼠中，总距离（活动过度的参数）和运动速度（冲动性）下降。实时荧光定量PCR结果显示，EE能增强杏仁核（Amy）CART反应，在Amy中观察到大量CART阳性细胞，尤其是杏仁中央核（CeA）。CART共定位于对小清蛋白和钙视网膜蛋白无免疫反应的GABA能细胞。EE诱导CART在CeA中的表达，其共定位于GABA能神经元，可能与SHR的多动和焦虑样行为减少有关。

To investigate the relation between environmental enrichment (EE) during the period of development and formation of emotion, spontaneously hypertensive rat (SHR) were allowed to EE for 5 weeks from P25, followed by assessment of emotional behavior and analysis in dopaminergic system focusing on cocaine and amphetamine regulated transcript (CART).In open-field test, total distance (parameter for hyperactivity) and locomotion speed (impulsivity) were deceased in EE-grown rats. In cylinder test, more quick adaptation to novelty was shown in EE. CART is enhanced by EE in the amygdala (Amy) by real-time PCR, and many CART-positive cells was observed in the Amy, especially in the central nucleus of amygdale (CeA). CART colocalized in GABAergic cells that were not immnoreactive to parvalbumin and calretinin. Data suggested that EE induced CART expression in CeA that colocalized in GABAergic neurons probably relating to decreased hyperactivity and anxiety-like behavior in SHR.