Involvement of phosphorylated L1-CAM in the plastic changes of nociecptive circuit following peripheral nerve injury

磷酸化L1-CAM参与周围神经损伤后伤害感受回路的可塑性变化

• 批准号: 23500418
• 负责人: YAMANAKA Hiroki
• 金额: $ 3.33万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23500418/
• 关键词: 神経傷害性疼痛; 脊髄後角; 可塑性; 細胞間接着因子; Casein kinase 2; 細胞間接着; シナプス可塑性; 神経障害性疼痛; L1-CAM; casein kinase 2; 神経因性疼痛; 後根神経節; シナプス

We examined whether peripheral nerve injury affects on the phosphorylation of L1-CAM in the rat DRG and spinal cord using a phospho-specific L1-CAM (Ser1181) antibody. Expression of phosphorylated L1-CAM was examined in rats (Sprague-Dawley, 250 g) that received tibial and common peroneal nerve ligation. Total and phosphorylated L1-CAM immunoreactivity (ir) were constitutively expressed in small un-myelinated DRG neurons. Nerve injury decreased total L1-CAM ir in the cytoplasm of somata in small neurons and increased it in cell membrane resulting to the formation of L1-CAM ir ring-like structures of in the injured small DRG neurons. Phosphorylated L1-CAM ir showed a dramatic decrease in the injured DRG. In dorsal horn, total L1-CAM showed the increase in the terminal of primary afferents horn. In contrast to the DRG, nerve injury increased the phosphorylated L1-CAM in the terminal of primary afferent in the dorsal horn .

我们使用磷酸化特异性L1-CAM（Ser 1181）抗体检测周围神经损伤是否影响大鼠DRG和脊髓中L1-CAM的磷酸化。在接受胫骨和腓总神经结扎的大鼠（Sprague-Dawley，250 g）中检测磷酸化L1-CAM的表达。总的和磷酸化的L1-CAM免疫反应性（ir）组成型表达在小的无髓鞘DRG神经元。神经损伤后，小神经元胞体内L1-CAM免疫阳性物质减少，胞膜内L1-CAM免疫阳性物质增多，形成L1-CAM免疫阳性环状结构。 磷酸化的L1-CAM免疫反应在受损的DRG中显示显著减少。在背角，L1-CAM总量在初级传入角终末增加。与DRG相比，神经损伤后，背角初级传入纤维终末磷酸化L1-CAM增加。

项目成果

脊髄microgliaに対するRhoキナーゼ阻害剤の影響と神経傷害性疼痛への関与

Rho 激酶抑制剂对脊髓小胶质细胞的影响及其参与神经性疼痛

• 发表时间: 2014
• 作者: 山中博樹;巽恵美子;小林希実子;阪上雅史;野口光一.
• 通讯作者: 野口光一.

Expression of leukotriene receptors in the rat dorsal root ganglion and the effect on pain behaviors.

大鼠背根神经节白三烯受体的表达及其对疼痛行为的影响。

• 发表时间: 2011
• 作者: Okubo M;Yamanaka H;Kobayashi K;Fukuoka T;Dai Y Noguchi K
• 通讯作者: Dai Y Noguchi K

Phosphorylation of L1-CAM is differentially regulated in somata and central terminal of DRG neuron after nerve injury

神经损伤后 DRG 神经元胞体和中枢末端 L1-CAM 磷酸化的差异调节

• 发表时间: 2012
• 作者: Yamanaka H;Kobayashi K;Noguchi K
• 通讯作者: Noguchi K

Expression of PGD2 and TXA2 synthases and the receptors in spinal cord following peripheral nerve injury. The 41th Annual Meeting of the Society for Neuroscience

周围神经损伤后脊髓中 PGD2 和 TXA2 合酶及其受体的表达。

• 发表时间: 2011
• 作者: Kanda H;Kobayashi K;Yamanaka H;Noguchi K
• 通讯作者: Noguchi K

Up-regulation of platelet-activating factor synthases and its receptor in spinal cord contribute to development of neuropathic pain following peripheral nerve injury.

• DOI: 10.1186/1744-8069-8-8
• 发表时间: 2012-02-02
• 期刊: Molecular pain
• 影响因子: 3.3
• 作者: Okubo M;Yamanaka H;Kobayashi K;Kanda H;Dai Y;Noguchi K
• 通讯作者: Noguchi K