Involvement of phosphorylated L1-CAM in the plastic changes of nociecptive circuit following peripheral nerve injury

我们使用磷酸化特异性L1-CAM （Ser1181）抗体检测周围神经损伤是否影响大鼠DRG和脊髓L1-CAM的磷酸化。在大鼠（Sprague-Dawley, 250 g）接受胫骨和腓总神经结扎后，检测磷酸化L1-CAM的表达。总和磷酸化L1-CAM免疫反应性（ir）在小的无髓鞘DRG神经元中组成性表达。神经损伤使小神经元胞质中L1-CAM ir总量减少，细胞膜中L1-CAM ir总量增加，导致损伤后小DRG神经元形成L1-CAM ir环状结构。磷酸化的L1-CAM ir显示损伤的DRG显著降低。在背角，总L1-CAM在初级传入角末端增加。与DRG相反，神经损伤增加了背角初级传入神经末梢L1-CAM的磷酸化。

We examined whether peripheral nerve injury affects on the phosphorylation of L1-CAM in the rat DRG and spinal cord using a phospho-specific L1-CAM (Ser1181) antibody. Expression of phosphorylated L1-CAM was examined in rats (Sprague-Dawley, 250 g) that received tibial and common peroneal nerve ligation. Total and phosphorylated L1-CAM immunoreactivity (ir) were constitutively expressed in small un-myelinated DRG neurons. Nerve injury decreased total L1-CAM ir in the cytoplasm of somata in small neurons and increased it in cell membrane resulting to the formation of L1-CAM ir ring-like structures of in the injured small DRG neurons. Phosphorylated L1-CAM ir showed a dramatic decrease in the injured DRG. In dorsal horn, total L1-CAM showed the increase in the terminal of primary afferents horn. In contrast to the DRG, nerve injury increased the phosphorylated L1-CAM in the terminal of primary afferent in the dorsal horn .