Development of ratiometric molecular probes for monitoring oxygen levels in living cells

开发用于监测活细胞中氧气水平的比率分子探针

• 批准号: 23500511
• 负责人: YOSHIHARA TOSHITADA
• 金额: $ 3.41万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23500511/
• 关键词: りん光; レシオ; 蛍光; 酸素; イリジウム錯体; 細胞

Ratiometric molecular probes for the measurement of oxygen levels in lipid membranes and living cells were designed and synthesized. The synthesized molecules showed an oxygen-insensitive fluorescence with coumarin 343 and an oxygen-sensitive phosphorescence with iridium complexes. The ratio of the phosphorescence intensity for the fluorescence intensity increased with the decrease of oxygen concentration in the solution under the presence of lipid membranes. These probes were added to the cell medium and cells were incubated. The fluorescence and phosphorescence images of living cells were obtained by using microscopic techniques. These results exhibited that the ratiometric molecular probe has a high advantage for the measurements and the images of oxygen levels in living cells.

设计并合成了一种用于测量脂膜和活细胞中氧含量的比率分子探针。合成的分子对香豆素343表现出氧不敏感的荧光，与Ir络合物表现出氧敏感的磷光。在有脂膜存在的情况下，随着溶液中氧浓度的降低，磷光强度与荧光强度的比值增大。将这些探针加入细胞培养液中，孵育细胞。用显微技术获得活细胞的荧光和磷光图像。这些结果表明，比率分子探针在活体细胞内氧水平的测量和图像方面具有很高的优势。

项目成果

細胞・組織内酸素濃度測定のための高感度近赤外りん光イリジウム錯体

高灵敏近红外磷光铱络合物，用于测量细胞和组织中的氧浓度

• 发表时间: 2013

Developments of Hypoxia-Sensing Probes with High Cellular Uptake Efficiency

具有高细胞摄取效率的缺氧传感探针的开发

• 发表时间: 2011
• 作者: T. Yoshihara;K. Ichikawa;M. Hosaka;T. Takeuchi;S. Tobita
• 通讯作者: S. Tobita

新規化合物およびそれを含む機能性発光プローブ

新化合物和含有它们的功能性发光探针

• 发表时间: 2014

新規錯体化合物, 並びにそれを用いた酸素濃度測定試薬及び癌の診断薬

新型复合化合物、氧浓度测定试剂以及使用其的癌症诊断剂

• 发表时间: 2013

Iridium Complex Probes for Monitoring of Cellular Oxygen Levels and Imaging of Hypoxic Tissues

用于监测细胞氧水平和缺氧组织成像的铱复合物探针

• DOI: 10.1117/12.910170
• 发表时间: 2012
• 期刊: Proc.SPIE
• 作者: Yoshihara;T.;Kobayashi;A.;Oda;M.;Hosaka;M.;Takeuchi;T.;Tobita;S.
• 通讯作者: S.